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建立一个高效和多功能基因组编辑平台,用于L. ruthenicum

Ying Sun¹, Zheng Hong¹, Wenwen Wang¹

¹Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development, Shanghai Engineering Research Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.

Plant physiology

|October 6, 2025

概括

这项研究开发了黑色古吉 (Lycium ruthenicum) 的先进基因组编辑工具,使得高效的基因淘汰和基基编辑成为可能. 这些工具将加速功能基因组研究和改善这种有价值的药用植物的特征.

科学领域:

植物生物技术植物生物技术
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

黑色古吉 (Lycium ruthenicum) 是一种有价值的功能性食品和药用植物,富含营养.
有限的遗传工具阻碍了功能性基因组研究和L. ruthenicum的特征改善.
优化基因转换对于推动这种物种的研究至关重要.

研究的目的:

建立一个强大的基因组编辑工具包,用于Lycium ruthenicum.
为了实现高效的基因淘汰和基因编辑功能分析.
为了促进黑色戈吉果的分子繁殖和特征改善.

主要方法:

优化了L. ruthenicum的基因转换系统,具有高效率 (95.4%).
开发了用于基因淘汰的集群定期间隔的短Palindromic重复/CRISPR相关蛋白9 (CRISPR/Cas9).
已建立的腺基编辑 (ABE) 系统,包括一个PAM放松变体 (SpRY) 和一个多重ABE系统.

主要成果:

使用CRISPR/Cas9.9实现了LrPDS (68.8%) 和LrBADH2 (96.0%) 的高编辑效率.
在LrPDS位置证明了72.2%的ABE效率.
在使用SpRY (5.3%) 和同时多重编辑 (33.3%) 的非正规NAN PAM站点成功执行了A-to-G编辑.

结论:

一个强大的基因组编辑工具包L. ruthenicum已经成功建立.
开发的工具显著提升了功能基因分析和分子繁殖能力.
这项工作为改善这一经济重要物种提供了必要的资源.

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