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对SARS-CoV-2的毒性研究尖蛋白在Bacillus subtilis子上表达

Atiqah Hazan¹, Amalia A Saperi¹, Nurfatihah Zulkifli¹

¹Tropical Infectious Diseases Research & Education Centre (TIDREC), Universiti Malaya, Kuala Lumpur, 50603, Malaysia.

BMC microbiology

|October 6, 2025

概括

在口服后,表达SARS-CoV-2尖端蛋白的重组细菌细菌子在小鼠中没有显示出毒性. 这支持它们作为安全的口服疫苗候选人对抗COVID-19的潜力.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
疫苗学疫苗学疫苗学

背景情况:

SARS-CoV-2尖端蛋白质是COVID-19疫苗的一个关键目标.
需要安全有效的输送系统来诱导全身和粘膜免疫.
细菌细菌子是显示抗原的稳定和安全平台.

研究的目的:

为了评估表达SARS-CoV-2尖端蛋白的复合Bacillus subtilis子的急性口服毒性.
评估这个平台对于潜在的口服疫苗开发的安全性.

主要方法:

在运动过程中,SARS-CoV-2 S1 域尖蛋白在 B. subtilis 子上得到表达.
在实验室中进行了细胞毒性测试.
BALB/c小鼠在三天内接受了高剂量的复合子,并被监测了14天.
对重要器官进行了总体尸检,组织学和生物化学分析.

主要成果:

没有观察到体外细胞毒性.
接受高达3 × 10 ^ 11 CFU/小鼠的小鼠没有出现不良影响.
重要器官的组织病理学和生物化学参数保持在正常范围内,表明没有病理变化.

结论:

口服SARS-CoV-2S1尖端蛋白表达B. subtilis子在高剂量时在小鼠中耐受良好.
这些发现支持进一步研究重组细菌子作为SARS-CoV-2的口服疫苗候选者.

关键词:

这种细菌是 Bacillus subtilis.有急性毒性的急性毒性.这就是SARS-CoV-2病毒.尖蛋白质是一种尖蛋白质.疫苗疫苗疫苗疫苗

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