在Sporidiobolales和Trichosporonales中的核分裂表型
Keita Aoki1, Moriya Ohkuma2, Takashi Sugita3
1Laboratory of Yeast Systematics, Tokyo NODAI Research Institute, Tokyo University of Agriculture, Setagaya, Tokyo, Japan.
Microbiology spectrum
|October 7, 2025
概括
基本菌酵母中的核分裂通常发生在子细胞中. 然而,这项研究发现,四种Trichosporonales物种,包括Trichosporon asahii,在母细胞内分裂细胞核,挑战现有的教条.
科学领域:
- * 菌群学 * 菌群学
- * 细胞生物学 * 细胞生物学
- * 遗传学 在遗传学方面
背景情况:
- * 基本菌酵母传统上在从母细胞核迁移后在子细胞内表现出核分裂.
- * 这与在Saccharomyces cerevisiae (一种ascomycetous酵母) 中观察到的核分裂过程形成鲜明对比.
- *所有物种中典型的基菌细胞核分裂模式的普遍性仍然不确定.
研究的目的:
- *研究基底菌菌酵母中核分裂模式的多样性,特别是Sporidiobolales和Trichosporonales类.
- * 识别那些偏离已确立的关于子细胞中发生的核分裂教条的物种.
- *对Trichosporon asahii的核分裂进行深入分析,该物种被确定为潜在的例外.
主要方法:
- *对51种Sporidiobolales和33种Trichosporonales酵母菌种进行核分裂表型的选.
- *利用4',6-二胺-2-英 (DAPI) 染色来可视化核DNA并识别关键的表型.
- *活细胞成像Trichosporon asahii表达素H2A蛋白标记mCherry实时观察核分裂.
主要成果:
- *四种Trichosporonales物种,包括Apiotrichum domesticum,Cutaneotrichosporon arboriforme,Trichosporon asahii和Vanrija humicola,显示了典型的子细胞核分裂表型的频率降低.
- *所有测试的Sporidiobolales物种都表现出传统的核分裂模式.
- *实时成像证实,Trichosporon asahii在母细胞内或在芽部分裂其核,没有先前迁移到子细胞. 在T. asahii中,核分裂位置与子细胞长度相关.
结论:
- *这项研究确定了Trichosporon asahii和其他三种物种作为传统的基底菌细胞核分裂模式的例外.
- * 这一发现突显了基底菌菌类酵母中核分裂策略的显著多样性.
- *了解核分裂中的这些变异可以有助于更好地理解真菌病原和进化.
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