花粉水化及其对人类支气管上皮细胞的诱导作用:对三种物种进行比较分析
Dimple Pathania1, Sudharsun Venkatesan2, Ivan Kourtchev3
1School of Engineering, Deakin University, VIC, 3216, Australia; Centre for Agroecology, Water and Resilience (CAWR), Coventry University, Ryton-on Dunsmore, CV8 3LG, United Kingdom.
The Science of the total environment
|October 7, 2025
概括
花粉破裂和颗粒生成因物种而异,影响过敏疾病的潜力. 草花粉独特地增加了与过敏原相关的活性氧物种 (ROS),在不同物种中不同影响细胞活力.
科学领域:
- 环境科学 环境科学
- 过敏和免疫学 过敏和免疫学
- 细胞生物学 细胞生物学
背景情况:
- 全球由花粉引起的过敏性疾病的增加.
- 气候变化可能会加剧花粉季节和花粉负载.
- 需要了解特定物种花粉的过敏性潜力.
研究的目的:
- 研究草,草和松花粉的水分诱导的破裂行为.
- 使用体外细胞模型评估这些花粉物种的过敏性潜力.
- 确定细胞对花粉提取物的反应,包括物理和生物化学性质.
主要方法:
- 在体外模型使用人类支气管上皮细胞 (Calu-3).
- 液化实验,以评估花粉破裂的倾向.
- 雾化以产生花粉气溶.
- 细胞活力测定和反应性氧物种 (ROS) 测量.
- 第5组草过敏原的量化 (Phl p 5).
主要成果:
- 观察到特定物种的花粉破裂模式;瑞草的破裂倾向最高.
- 草花粉产生了大量的可呼吸的子花粉颗粒.
- 松花粉显著降低了细胞活力,而花粉的影响最小.
- 草花粉独特地诱导了大量的ROS产量,与增加的Phl p 5水平相关.
- 牛花粉保持高细胞活力,只有轻微的ROS增加,这表明了其他机制.
结论:
- 花粉的物理特性 (破裂,颗粒大小) 和生物化学特性 (ROS,过敏原含量) 影响过敏潜力.
- 发现的特定物种的细胞反应对临床管理有影响.
- 这些发现为了解气候变化中的花粉-过敏相互作用提供了基础.
更多相关视频
08:42Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures
Published on: May 26, 2023
4.0K
07:07Author Spotlight: A High-Resolution, Single-Grain, In Vivo Pollen Hydration Bioassay for Arabidopsis thaliana
Published on: June 30, 2023
3.3K
相关概念视频
01:28Asthma I: Introduction
311
Asthma is a chronic inflammatory disorder of the airways characterized by variable airflow obstruction and heightened bronchial responsiveness to a wide range of triggers. The underlying inflammation leads to airway swelling, mucus hypersecretion, and smooth muscle constriction, all of which narrow the airway lumen and impede airflow. Clinically, asthma presents with recurrent episodes of wheezing, shortness of breath, chest tightness, and coughing, symptoms that typically vary in intensity and...
311
01:24Chronic Obstructive Pulmonary Disease III: Chronic Bronchitis Features
77
Chronic bronchitis is a key phenotype of chronic obstructive pulmonary disease (COPD), characterized by airway-centered inflammation and mucus overproduction. It develops from long-term exposure to harmful particles or gases, most commonly cigarette smoke, which triggers a persistent inflammatory response.Cellular and Structural ChangesInflammation initially affects the large bronchi and later the smaller airways, with infiltration by immune cells, including neutrophils, macrophages, and...
77