父亲的运动通过精子的微型RNA赋予后代的耐力能力
Xin Yin1, Azhar Anwar2, Linbo Yan2
1Department of Andrology, Nanjing Drum Tower Hospital, State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), School of Life Sciences, Nanjing University, Nanjing, China; The Second People's Hospital of Changzhou, The Third Affiliated Hospital of Nanjing Medical University, Changzhou Medical Center, Nanjing Medical University, Changzhou, China; State Key Laboratory of Reproductive Medicine and Offspring Health, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China; Pingshan Translational Medicine Center, Shenzhen Bay Laboratory, Shenzhen, China; PKU-HKUST ShenZhen-HongKong Institution, Shenzhen, China.
父亲的运动可以提高后代的运动能力和代谢健康. 精子微RNA通过调节胚胎基因表达来调节这些效应,促进线粒体功能.
科学领域:
- 运动生理学 运动生理学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 线粒体生物学 线粒体生物学
背景情况:
- 众所周知,父亲的运动会影响后代的特征,但机制尚不清楚.
- 过氧体增殖器激活受体 γ 协同激活器-1α (PGC-1α) 对于线粒体功能和运动适应至关重要.
- 精子中的小RNA与跨代遗传有关.
研究的目的:
- 研究父亲运动影响后代运动能力和代谢健康的机制.
- 确定PGC-1α和精子微RNA在调解这些父性影响中的作用.
- 阐明涉及传递运动诱导适应的分子途径.
主要方法:
- 在小鼠中,比较运动训练与久坐不动的父亲的后代.
- 使用具有肌肉特异性PGC-1α过度表达的转基因小鼠.
- 进行精子小RNA注射到卵子中.
- 分析后代的行为,代谢和分子表型.
主要成果:
- 运动训练父亲的后代表现出改善的运动能力和代谢参数.
- 父亲的肌肉特异性PGC-1α过度表达给后代带来了类似的好处.
- 来自炼过的父亲的精子小RNA在后代中重现了这些有益的表型.
- 运动训练和PGC-1α精子微RNA重塑抑制了胚胎NCoR1,增强了线粒体生物发生.
结论:
- 父亲的运动通过精子介导的表观遗传机制诱导后代的有益适应.
- 精子微RNA在通过调节胚胎NCoR1表达来传递父亲运动效应方面发挥着至关重要的作用.
- 这项研究突出了通过PGC-1α和microRNAs对子女代谢健康和运动表现的父亲影响的新途径.
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