开发可调整的硬质和软质晶格支架,用于多规模组织工程应用
Jasmine Carpenter1, Elijah Barnes1, Amrita Natarajan1
1Laboratory for Polymeric Biomaterials, Department of Biomedical and Mechanical Engineering, Alabama State University, Montgomery, Alabama 36104, United States.
ACS applied bio materials
|October 8, 2025
概括
研究人员使用3D打印开发了可调节的硬和软格子支架. 表面修饰和材料选择增强了组织工程应用的细胞附着和骨形成.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 可调节的支架对于多尺度组织工程至关重要.
- 设计具有可控机械性能和生物活性的支架对于成功的组织再生至关重要.
研究的目的:
- 用于计算设计和3D打印可调节的硬和软格子支架.
- 研究格子拓,材料组成和表面修饰对机械性质和细胞反应的影响.
- 建立一个框架,用于创建复杂组织再生的混合脚手架系统.
主要方法:
- 通过计算设计和3D打印的多乳酸 (PLA) 陀螺和钻石脚手架,具有不同的参数.
- 通过压缩测试表征机械性能,通过微型CT测试表征架构.
- 用聚多巴胺 (PDA) 进行表面修改的PLA支架.
- 通过用酸 (HAP) 纳米晶体强化酸水凝和3D生物打印各种几何形状来开发软支架.
- 评估细胞附着,扩散,增殖和骨质分化 (hMSCs) 以及代谢活性/DNA含量 (纤维细胞).
主要成果:
- 在PLA的陀螺和钻石支架上,可调节的机械性能 (模块为82-405 MPa) 和可控的孔隙性 (63-85%).
- PDA表面修饰增强了人间介质干细胞 (hMSC) 的附着和骨质分化,PDA涂层的钻石支架显示出最高的沉积量.
- 软酸盐-HAP支架在增加HAP含量时显示出更好的打印能力.
- 纤维细胞研究显示,方形蜂软支架中代谢活性和DNA含量最高.
结论:
- 格子几何学,材料强化和表面生物功能化可以系统地结合起来,创建可调节的支架.
- 这些可调节的支架对承载和软组织应用都非常有希望.
- 这些发现为复杂组织再生的空间和机械梯度的混合脚手架系统奠定了基础.
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