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在STEMI患者中识别与血小板功能相关的基因
Jingjing Zhu1, Shuangya Yang1, Qing Guo1
1Department of Cardiology, Affiliated Hospital of Zunyi Medical University, Zunyi, China.
Frontiers in genetics
|October 8, 2025
概括
这项研究确定了MAPK3和GRB2作为诊断ST升高心肌梗塞 (STEMI) 的关键血小板功能相关基因. 这些生物标志物对STEMI患者具有显著的诊断价值和临床相关性.
科学领域:
- 生物标志物发现发现
- 心血管研究研究心血管研究
- 分子诊断学 分子诊断学
背景情况:
- ST升高心肌梗塞 (STEMI) 涉及由血小板活动驱动的显著心肌损伤.
- 确定STEMI可靠的生物标志物对于及时诊断和治疗至关重要.
- 血小板功能在STEMI的发病过程中起着至关重要的作用.
研究的目的:
- 确定ST升高心肌梗塞 (STEMI) 的新型血小板功能相关生物标志物.
- 评估这些已识别的生物标志物在STEMI患者的诊断潜力.
主要方法:
- 对基因表达数据集 (GSE59867和GSE123342) 的分析,以确定差异表达的血小板功能相关基因 (DEPRGs).
- 构建蛋白质-蛋白质相互作用 (PPI) 网络并识别枢纽基因.
- 使用接收器操作特征 (ROC) 分析和定量实时聚合酶链反应 (RT-qPCR) 验证诊断性能.
主要成果:
- 确定了245种DEPRG,主要涉及血液静止和血小板激活.
- 确定了11个枢纽基因,其中GRB2,MAPK1,MAPK3,PIK3CA,AKT1和PIK3R1显示出强大的诊断潜力 (AUC>0.7).
- 在独立的数据集和实验模型中,MAPK3和GRB2被验证为关键的诊断生物标志物,具有显著的诊断价值 (AUC>0.7).
结论:
- MAPK3和GRB2被确定为STEMI的有前途的血小板功能相关生物标志物.
- 这些基因对STEMI具有显著的诊断准确性和临床相关性.
- 这些发现有助于开发STEMI的新型诊断策略.
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