在城市农业系统中,与绿叶蔬菜和土壤相关的细菌的多药性耐药性
Erin Harrelson1, Qingyue Zeng1, Mairui Gao1
1Department of Nutrition and Food Science, University of Maryland, College Park, MD, United States.
Frontiers in plant science
|October 8, 2025
概括
城市农场可以容纳多种耐药细菌,对食品安全构成风险. 这项研究分析了城市农场细菌,发现常见的土壤微生物携带耐药性基因和移动元素,突出了潜在的传播风险.
科学领域:
- 环境微生物学环境微生物学
- 食品安全 食品安全
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 城市农场和社区花园对于当地食品至关重要,但也可以成为新出现的污染物的储,例如抗菌素耐药性 (AMR).
- 之前的研究发现了来自华盛顿特区叶蔬菜生产环境的100多种多药耐药 (MDR) 细菌分离物.
研究的目的:
- 在87种来自城市叶绿植物,根区土壤和散土的MDR细菌分离物上进行全基因组测序.
- 综合描述MDR基因型,包括分类学,抗菌素耐药性基因 (ARG),应激反应基因和移动遗传元素 (MGE).
主要方法:
- 87个MDR细菌分离物的全基因组测序 (WGS).
- 生物信息分析用于识别细菌分类,抗微生物耐药性基因 (ARG),应激反应基因和移动遗传元素 (MGE).
- 对ARG地点相对于MGE进行分析,以评估动员的潜力.
主要成果:
- MDR分离物属于4个属和14个属,主要包括*Pseudomonas*,*Serratia*,*Providencia*和*Bacillus*.
- 主要的ARG赋予了对多药物排放,β-乳糖胺和四环素的耐药性.
- 所有MDR分离物都含有MGEs,在这些元素附近发现了19个ARG,这表明可能存在横向基因转移.
结论:
- 城市农业中的MDR细菌,包括农产品上的MDR细菌,主要是常见的土壤相关的种类.
- 这些细菌具有多样化的ARG和MGE,表明在城市食品系统内AMR传播的潜在途径.
- 基因组表征为城市农业生态系统中MDR的遗传基础提供了洞察力.
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