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在子宫内粘附中与免疫相关的枢纽基因:一种生物信息学方法

Fengqing Lv¹, Sang Luo², Fengjuan Xu¹

¹College of the First Clinical Medicine, Ningxia Medical University, Yinchuan, Ningxia, China.

|October 8, 2025

概括

宫内粘附 (IUA) 导致女性不孕. 这项研究确定了7个与免疫相关的基因,验证了3个并提出了4个IUA诊断和潜在治疗标的生物标志物.

科学领域:

生殖生物学生殖生物学
免疫学免疫学免疫学
计算生物学计算生物学

背景情况:

子宫内粘附 (IUA) 是获得女性不孕症的一个重要原因,通常是由于子宫内手术干预造成的.
IUA背后的分子机制,特别是免疫系统在子宫内膜修复中的失调作用,仍然不完全理解.
全面的机理学研究对于阐明IUA病原性至关重要.

研究的目的:

通过计算识别和实验验证与子宫内粘附 (IUA) 病变发生相关的分子特征.
探索免疫失调在导致IUA的子宫内膜修复过程中的作用.
确定IUA潜在的诊断生物标志物和治疗点.

主要方法:

使用GEO2R进行差异基因表达分析,对公开的子宫内膜转录基因数据集 (GSE224093) 进行系统的再分析.
与ImmPort数据库集成,通过基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析进行免疫上下文化和多层功能注释.
权重基因共同表达网络分析 (WGCNA) 以确定保存的共同表达模块,然后进行定量实时聚合酶连锁反应 (qPCR) 和枢纽基因的免疫组织化学 (IHC) 验证.

主要成果:

综合的多组学分析确定了七个点基因,这些基因与IUA病原发生有关.
通过计算观察到TUBB3,WNT5A,GDF7,IGF1和BIRC5的下调,以及PTGDS和CCL14的上调.
实验验证证在人类和小鼠模型中证实了这些表达模式,在TUBB3和PTGDS中发现了特定物种的变异. 对IGF1,WNT5A,BIRC5和GDF7.7的蛋白质表达变化得到证实.

结论:

使用综合生物信息学识别了七个与IUA病变相关的免疫相关基因特征.
在实验验证后,三个基因 (TUBB3,PTGDS,CCL14) 显示出特定物种的表达模式.
四个基因 (WNT5A,IGF1,BIRC5,GDF7) 被提出为IUA诊断的潜在生物标志物,其中WNT5A和TGF-β通路基因表明治疗标.

关键词:

生物信息学分析不同表达的基因.与免疫相关的基因综合性分析是一种综合性分析.宫内粘附在子宫内粘附.

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