宿主介导的内菌-病原体在根部中的竞争使Arabidopsis thaliana无症状的真菌殖民成为可能
Kei Hiruma1,2,3, Kuldanai Pathompitaknukul1, Hiroyuki Tanaka4
1Department of Science and Technology, Nara Institute of Science and Technology, Nara 630-0192, Japan.
Plant & cell physiology
|October 8, 2025
概括
一种内性真菌,Collettotrichum fructicola (CfE),通过产生抗真菌代谢产物,可以预防Brassicaceae植物的疾病. 这种真菌与宿主相互作用,涉及托代谢物,突出了真菌与真菌在植物健康方面的竞争.
科学领域:
- 植物病理学 植物病理学
- 微生物生态学 微生物生态学
- 分子植物-微生物相互作用
背景情况:
- 种子植物通常是没有疾病的微生物的宿主,但防止病原体进展的机制尚不清楚.
- 内生菌可以为植物带来好处,但它们在抑制病原体中的作用尚未完全理解.
研究的目的:
- 研究一种内生菌菌,Collettotrichum fructicola (CfE) 抑制一种相关的病原体,C. gloeosporioides (CgP) 在布拉西卡植物中的机制.
- 阐明真菌与真菌相互作用和宿主代谢物在维持植物健康中的作用.
主要方法:
- 与CgP和CfE在Arabidopsis thaliana根中的共同注射实验.
- 在不同的注射条件下对植物根的转录组分析.
- 鉴定参与相互作用的真菌基因和宿主代谢物.
主要成果:
- 在Arabidopsis中,CfE共同殖民抑制了CgP诱导的根死.
- 在共注射过程中,超过700个CfE基因被特别诱导,在二次代谢途径中得到丰富.
- CfE的保护功能和内菌殖民依赖于宿主衍生的托基抗微生物代谢产物.
结论:
- 由CfE产生的抗真菌代谢物中介的真菌-真菌竞争对于无症状殖民和植物健康至关重要.
- 宿主衍生代谢物在调节内菌殖民和病原体抑制方面发挥作用.
- 这项研究揭示了一种通过微生物相互作用抑制植物疾病的新机制.
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