在室温下储存的棕酒腐烂过程中微生物群落的变化
Constant K Attchelouwa1, Marius D Akissi2, Claude G Z Kouadjo3
1Department of biochemistry-genetic, Training and Research Unit in Biological Sciences, Peleforo Gon Coulibaly University, BP 1328, Korhogo, Côte d'Ivoire. attchelouwa@gmail.com.
概括
这项研究确定了棕葡萄酒中关键的腐烂微生物,包括酵母,乳酸细菌 (LAB) 和酸细菌 (AAB). 了解这些微生物有助于制定扩大棕酒产量战略.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 发酵技术的发酵技术
背景情况:
- 棕酒是一种受欢迎的饮料,在储存过程中容易变质.
- 了解微生物动态对于延长保质期至关重要.
研究的目的:
- 为了识别室温储存的棕葡萄酒的腐烂微生物群.
- 确定延长棕葡萄酒保质期的策略.
主要方法:
- 酵母,乳酸细菌 (LAB) 和酸细菌 (AAB) 的微生物计数.
- 使用PCR-RFLP和测序进行物种识别.
- 通过下一代测序 (NGS) 进行细菌社区分析.
主要成果:
- 从生产商和再销售商的样本中,微生物计数在生产商和再销售商的样本之间存在差异.
- 已识别的酵母物种包括Saccharomyces cerevisiae和Brettanomyces bruxellensis.
- 在储存期间占主导地位的细菌是乳酸菌,齐莫莫纳斯和菌.
结论:
- 特定的微生物物种如Brettanomyces bruxellensis和Acetobacter oryzoeni在储存期间增加.
- 了解腐烂动态有助于开发棕葡萄酒的有针对性的保存方法.
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