从甘包水解盐中生产西龙酸,使用工程化Komagataella phaffii菌株
Isabela Castro de Almeida1,2, João Ricardo M Almeida2, Fabricio Machado1
1Instituto de Química, Universidade de Brasília, Campus Universitário Darcy Ribeiro, 70910-900, Brasília, DF, Brazil.
Applied biochemistry and biotechnology
|October 8, 2025
概括
这项研究优化了微生物西隆酸生产,使用了复合的Komagataella phaffii菌株. 作为共同基质的甘油显著提高了生物反应器培养中的西龙酸产量和生产力.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
- 生物化学工程 生物化学工程
背景情况:
- 锡龙酸是一种有价值的锡衍生物,用于食品,制药和化学工业.
- 与化学合成相比,微生物生产在安全性,环境友好性和温和的工艺条件方面具有优势.
- 从可再生资源有效地生产西隆酸对于可持续的工业应用至关重要.
研究的目的:
- 开发和优化一种复合的Komagataella phaffii菌株,用于从西洛中增强酸 (XA) 生产.
- 评估不同辅助基质 (葡萄糖与糖醇) 和发酵策略 (批量与食批量) 对XA生产的影响.
- 为了研究使用从甘包中提取的红纤维素解液来生产XA.
主要方法:
- 利用基因工程的Komagataella phaffii菌株进行西龙酸生物合成.
- 在软瓶和生物反应器中进行批量和料批量发酵,将葡萄糖和甘油作为共同基质进行比较.
- 采用脉冲养策略和计算机控制的生物反应器条件,以优化工艺.
- 评估了从甘包水解盐中提取的氧化甘的XA生产.
主要成果:
- 甘油作为共同基质的性能优于葡萄糖,导致更高的西龙酸标位和批发发酵的产量 (24.88 g.L-1).
- 在生物反应器中优化脉冲批次发酵实现了最大的西龙酸度为55.11g.L-1.1.
- 通过使用甘包水解糖的氧化,证明了42.30g.L-1的氧化酸的产生.
结论:
- 糖醇是一种优质的辅基质,可以增强重组K. phaffii. 的氧化酸产量.
- 先进的发酵策略,特别是生物反应器中的脉冲批发酵,显著提高了西龙酸产量和生产率.
- 证实了利用甘包水解盐用于可持续的西龙酸生产的可行性.
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