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相关概念视频

RNA Splicing01:32

RNA Splicing

60.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Pre-mRNA Processing: RNA Splicing01:36

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Conservative Site-specific Recombination and Phase Variation02:53

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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General Transcription Factors01:30

General Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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相关实验视频

Updated: Jan 15, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
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SRSF12是一种灵长类动物特有的拼接因子,可诱导组织特异的基因表达程序.

Jimmy Ly1,2, Sarah L Cady1, Sofia Haug1

  • 1Whitehead Institute for Biomedical Research, Cambridge, MA 02142.

Molecular biology of the cell
|October 8, 2025
PubMed
概括

剪接因子SRSF12在灵长类动物中高度表达,特别是在大脑和丸中. 它在人体细胞中的过度表达激活特定的基因并导致细胞循环停止,这表明灵长类动物在基因调节中的特定作用.

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 细胞生物学 细胞生物学

背景情况:

  • 替代拼接产生蛋白质组的多样性,由拼接因素,如胺/氨酸丰富 (SR) 蛋白调节.
  • SRSF12是SR蛋白家族的一个特征较差的成员,在脊椎动物中保存,但在大多数哺乳动物中表达较低.

研究的目的:

  • 研究拼接因子SRSF12的功能和表达模式.
  • 了解SRSF12在灵长类动物特异性基因调节和细胞过程中的作用.

主要方法:

  • 对不同物种和组织中SRSF12表达的分析.
  • 在SRSF12淘汰赛小鼠模型研究中.
  • 在培养的人类细胞中进行同局部化和相互作用研究.
  • 宫外SRSF12表达实验以评估转录和细胞效应.

主要成果:

  • 在SRSF12淘汰赛小鼠中,没有显著的生理或转录组变化.
  • 在灵长类动物中,SRSF12的表达更高,特别是在丸,卵细胞和大脑中.
  • 在人体细胞中异卵性SRSF12表达诱导了丸和大脑特异性基因激活,线粒离子停止和细胞死亡.
  • 现型需要RNA识别动机和SRSF12的C端域.

结论:

  • SRSF12已经演化出了灵长类动物特有的表达模式.
  • SRSF12在调节灵长类动物特异性基因方面发挥作用,特别是那些涉及半分裂,丸和大脑功能的基因.
  • SRSF12的功能与其独特的结构领域和灵长类动物特有的表达方式有关.