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来自SARS-CoV-2 Omicron BF.7变种RBD的抗体制备和免疫学分析

Quansheng Wang¹, Chenyi Wang¹, Zhicheng Zhang²

¹Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China; Nanchang City Key Laboratory of Animal Virus and Genetic Engineering, Nanchang, Jiangxi 330045, China; College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.

Molecular immunology

|October 8, 2025

概括

研究人员成功地在E. coli中产生了SARS-CoV-2 Omicron BF.7尖端蛋白受体结合域 (RBD). 这种蛋白质引起强烈的免疫反应,有助于检测Omicron变种和疫苗开发.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
生物技术是生物技术.

背景情况:

在SARS-CoV-2尖峰 (S) 蛋白对于病毒入口和COVID-19的严重程度至关重要.
在S蛋白受体结合域 (RBD) 中的突变驱动了Omicron变种的传播和免疫逃避.

研究的目的:

为SARS-CoV-2 Omicron BF.7 变异S蛋白 RBD.构建一个 prokaryotic 表达向量.
使用大肠杆菌表达和净化BF.7 RBD蛋白质.
为免疫学研究生成针对BF.7 RBD的多克隆抗体.

主要方法:

构建了pET-28a-BF.7-RBD表达向量的结构.
在大肠杆菌中蛋白质表达的优化 (温度,时间,IPTG度).
在小鼠中生成多克隆抗体,并通过ELISA评估抗体标位;评估细胞免疫反应 (IFN-γ).

主要成果:

在大肠杆菌中实现了pET-28a-BF.7-RBD结构的稳定表达.
在免疫小鼠中观察到高抗体标位 (高达1:204800).
生成的多克隆抗体检测到来自不同来源的RBD蛋白质,并刺激了细胞中高IFN-γ的产生.

结论:

在大肠杆菌中成功大规模生产免疫性Omicron BF.7 RBD蛋白质.
在小鼠中表现出强大的幽默和细胞免疫反应.
开发出高强度的多克隆抗体,对Omicron变种检测和疫苗研究有价值.

关键词:

奥米克朗 BF.7 病毒聚克隆抗体是一种多克隆抗体.Prokaryotic 的表达方式在RBD中,RBD是RBD.

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