Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.6K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Optimizing photoexcitation conditions for time-resolved X-ray solution scattering experiments.

FEBS open bio·2026
Same author

Assessing protein-specific radiation damage in time-resolved X-ray solution-scattering experiments at high-brilliance synchrotrons using fast detector readout.

Acta crystallographica. Section D, Structural biology·2026
Same author

Quantitative assessment of flow between cerebrospinal and interstitial fluid compartments in humans.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

3D-printed syringe holder with synchronized push-pull action.

HardwareX·2026
Same author

International Expert Opinion on Optimal Switching to Cladribine Tablets from Other High-Efficacy Disease-Modifying Therapies for Relapsing-Remitting Multiple Sclerosis: Opportunities and Challenges.

Neurology and therapy·2026
Same author

A light-triggered Time-Resolved X-ray Solution Scattering (TR-XSS) workflow with application to protein conformational dynamics.

FEBS open bio·2026

相关实验视频

Updated: Jan 15, 2026

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions
08:12

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

Published on: July 11, 2017

7.8K

在实时跟踪由结化合物触发的蛋白质动态中解决结构异质性

Konstantinos Magkakis1, Fredrik Orädd1, Christian Pett1

  • 1Department of Chemistry, Umeå University, Umeå, Sweden.

Journal of molecular biology
|October 8, 2025
PubMed
概括

研究人员使用时间解析的X射线溶液散射 (TR-XSS) 和分子动力学模拟研究了蛋白质构造变化. 他们开发了一种分析复杂蛋白质动态的方法,揭示了酶反应期间的中间状态和展开.

关键词:
腺酸乙激酶的使用方法整体优化整体优化遗传算法是一种遗传算法.蛋白质动力学 蛋白质动力学时间解析的X射线溶液散射.

更多相关视频

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.7K
Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools
10:41

Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

Published on: December 16, 2015

9.3K

相关实验视频

Last Updated: Jan 15, 2026

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions
08:12

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

Published on: July 11, 2017

7.8K
Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.7K
Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools
10:41

Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

Published on: December 16, 2015

9.3K

科学领域:

  • 生物化学 生物化学
  • 结构生物学 结构生物学
  • 生物物理学的生物物理.

背景情况:

  • 蛋白质采用多重构造,使结构研究复杂化.
  • 时间解析的X射线溶液散射 (TR-XSS) 监测蛋白质动态.
  • 中的化合物触发反应,但释放动力学和平衡状态带来了解释挑战.

研究的目的:

  • 为了应对TR-XSS数据解释中的挑战,由于非即时的结化合物释放.
  • 在催化循环期间建模和可视化蛋白质结构异质性.
  • 建立一个分析快速蛋白质构造变化的框架.

主要方法:

  • 使用大肠杆菌腺酸激酶 (AdK) 作为模型系统.
  • 采用了基于同步的时间解析X射线溶液散射 (TR-XSS).
  • 集成分子动力学 (MD) 模拟用于基于集体的结构改进.

主要成果:

  • 在平衡状态下确定了中间蛋白质构造,具有部分开放/关闭的域.
  • 观察到的转向完全封闭/开放的形状和在ATP可用性时部分展开.
  • 证明非瞬间基质释放影响过渡动力学.

结论:

  • 基于集体的结构改进有效地应对非瞬间基板释放带来的挑战.
  • 蛋白质构造变化在酶反应中遵循裂变模型.
  • 这项研究为剖析快速,体化合物诱导的蛋白质动态提供了一个框架.