相关实验视频

Updated: Jan 15, 2026

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Published on: March 17, 2023

circ-0007006通过稳定HBEGF表达在体外减弱甲状腺相关眼病的纤维化

Zhihui Xu¹, Xiaoli Bao¹, Anqi Sun¹

¹State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou, China.

Experimental eye research

|October 8, 2025

概括

循环RNAcirc-0007006在纤维化甲状腺相关眼科病 (TAO) 中被减少. 它通过向miR-383-3p/HBEGF通路来抑制纤维化,为TAO提供了潜在的治疗标.

科学领域:

眼科医生眼科眼科
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

甲状腺相关的眼病 (TAO) 是一种自身免疫轨道疾病.
纤维化是II型TAO的一个关键不可逆转的特征,治疗选择有限.
循环RNAs (circRNAs) 与疾病有关,但它们在TAO中的作用尚不清楚.

研究的目的:

为了研究circRNAs在纤维性TAO中的作用.
为了确定治疗纤维化TAO的潜在分子标.

主要方法:

高通量RNA测序以识别TAO组织中差异表达的circRNA.
在轨道纤维细胞中进行功能测试,以评估hsa_circ_0007006在纤维化中的作用.
机械研究涉及RNA测序,miRNA分析和西式涂抹.
救援实验以验证治疗潜力.

主要成果:

Hsa_circ_0007006在纤维化TAO组织中显著下调.
Circ-0007006通过降低COL1A1,α-SMA,HAS1和HAS2的调节和抑制SMAD2/3酸化来抑制纤维化.
Circ-0007006作为对 miR-383-3p 的竞争性内源RNA,稳定HBEGF的表达.
外源的HBEGF逆转了circ-0007006 knockdown的亲纤维效应.

结论:

环-0007006/miR-383-3p/HBEGF轴是TAO纤维化中一种新的调节途径.
Circ-0007006是纤维化TAO的潜在治疗标.

关键词:

纤维化纤维化纤维化根据HBEGF的说法,甲状腺相关的眼睛疾病.环RNA 环RNA 是一个环RNA.在 miR-383-3p 中.

更多相关视频

An Ex vivo Culture System to Study Thyroid Development

An Ex vivo Culture System to Study Thyroid Development

Published on: June 6, 2014

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

相关概念视频

Synthesis and Regulation of Thyroid Hormones

Synthesis and Regulation of Thyroid Hormones

Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...

Functions of Thyroid Hormones

Functions of Thyroid Hormones

The thyroid hormone (TH) plays a pivotal role in the intricate orchestration of physiological processes, exerting profound effects on development, metabolism, and homeostasis throughout different life stages.
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...