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微生物全细胞生物催化剂用于从纳林根宁生产罗

Siqi Ding^1,2, Jing Chen^1,2, Yongjie Wang^1,2

¹State Key Laboratory of Synthetic Biology, Frontiers Science Center for Synthetic Biology, School of Synthetic Biology and Biomanufacturing, Tianjin University, Yaguan Road 135, Jinnan District, Tianjin 300350, China.

ACS synthetic biology

|October 8, 2025

概括

研究人员开发了一种新方法,使用一种细菌Lactobacillus plantarum生产罗. 这种生物转化途径提供了一种更有效的方法来制造用于制药和营养药的罗.

科学领域:

生物技术是生物技术.
微生物工程微生物工程
代谢工程是代谢工程.

背景情况:

罗是一种二甲,具有重要的药物和营养价值.
传统的植物提取法不有效.
需要一种基于微生物的生产系统来生产素.

研究的目的:

为了建立一个生物转化途径,从Lactobacillus plantarum中从纳灵宁中生产罗.
为了优化工程途径以增加罗的产量.
为了证明使用L. plantarum作为黄类生物合成的主体的可行性.

主要方法:

选异构的铁硫含有酸酸酶.
弗拉减少酶和形成脱酶的同时表达,用于辅因子再生.
促进器工程,铁吸收基因过度表达和铁度优化.
酸还原酶的结构导向半导体设计.

主要成果:

在L. plantarum中成功建立了一种新型的生物合成路径,用于生产花素.
实施了双NADH-FAD辅因子再生系统.
工程L. plantarum取得了70.53毫克/升的花素标位.
鉴定出了酸乙酸还原酶的D656R突变,增强了罗的产生.

结论:

植物乳杆菌 (Lactobacillus plantarum) 是一种可行的,有前途的宿主,用于罗的生物合成.
开发的生物转化途径显著提高了罗的生产效率.
这项研究代表了第一份关于L. plantarum中黄类生物合成的报告,为生产罗衍生物开辟了道路.

关键词:

乳酸菌植物植物 (Lactobacillus plantarum) 是一种植物菌.辅助因子再生的复原酸可以降低酸酶.在那里,有个叫做naringenin的东西.佛罗里丁是一种色素.合成生物学合成生物学

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