聚乙烯微塑料被人类牙纤维细胞内化,增强细胞运动性并诱导通过蛋白质组分析揭示的分子变化
Federica Di Cintio1,2, Anna Giulia Ruggieri2,3, Chiara De Simone1
1Department of Oral, Medical and Biotechnological Sciences, University "G. d'Annunzio" of Chieti-Pescara, Chieti, Italy.
Scientific reports
|October 8, 2025
概括
聚烯微塑料 (PS-MPs) 进入人类牙纤维细胞,改变细胞功能. 这项研究揭示了PS-MPs破坏关键通路,影响细胞运动和存活,表明潜在的健康风险.
科学领域:
- 环境科学 环境科学
- 细胞生物学 细胞生物学
- 毒理学 毒理学 毒理学
背景情况:
- 塑料污染,特别是微塑料 (MPs),构成了全球环境和健康威胁.
- 聚钢 (PS) 是一种常见的塑料,而基于PS的MP (PS-MP) 是环境污染物.
- 暴露于PS-MPs与细胞损伤有关,需要进一步调查.
研究的目的:
- 研究1μmPS-MPs对人类牙纤维细胞 (hGF) 的影响.
- 探索PS-MPs对hGF细胞的细胞吸收和生物影响.
主要方法:
- 人类牙纤维细胞 (hGF) 暴露于1微米PS-MPs.
- 使用共聚焦显微镜,传输电子显微镜 (TEM) 和流细胞计,评估了细胞吸收.
- 进行蛋白质组分析以确定蛋白质表达和细胞通路的变化.
主要成果:
- 在测试度和时间点上,PS-MPs显示出有限的细胞毒性.
- 大约10%的hGF细胞内化了PS-MPs.
- 蛋白质组分析揭示了蛋白质静止的显著变化,包括代谢,内分泌功能,炎症反应和上皮-介质酶过渡的干扰.
- 被治疗的细胞表现出增加的运动性.
结论:
- 这些PS-MP被hGF细胞内化.
- 暴露于PS-MP显著影响涉及炎症,运动和生存的细胞通路.
- 这些发现凸显了PS-MP暴露对人类健康的潜在风险.
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