多代气温对欧洲海的父系表观遗传的影响
Núria Sánchez-Baizán1,2, François Allal2, Marc Vandeputte2
1Institut de Ciències del Mar (ICM), Spanish National Research Council (CSIC), Barcelona, Spain.
Epigenetics & chromatin
|October 8, 2025
概括
温度变化可以改变DNA甲基化模式,影响基因表达和适应. 欧洲海的一些环境诱导的表观遗传变化被传给后代,显示出多代表观遗传.
科学领域:
- 环境表观遗传学环境表观遗传学
- 基因组学就是基因组学.
- 进化生物学是进化的生物学.
背景情况:
- 环境变化,如温度变化,可以诱导表观遗传修饰,影响基因表达,表型和适应.
- 欧洲海是研究表观遗传反应的有价值的模型生物,因为它具有混合的遗传和环境性别决定.
研究的目的:
- 为了研究温度如何影响欧洲海的全基因组DNA甲基化模式.
- 为了确定温度诱导的表观遗传修饰,特别是性发育基因,是否可以跨代传播.
- 区分祖先 (父母) 和发育 (后代) 温度对DNA甲基化的影响.
主要方法:
- 减少表示双硫酸盐测序 (RRBS) 用于分析欧洲海两代 (F0和F1) 的单核酸分辨率的DNA甲基化.
- 父母鱼 (F0) 被暴露在控制 (16°C) 或升高 (21°C) 的温度下,其后代 (F1) 被 subjected to四种不同的热法.
- 根据其与父母 (F0),后代 (F1) 或交互性 (F0 x F1) 温度效应的关联,确定和分类了差异甲基化区域 (DMR).
主要成果:
- 显著比例的DMR (37%在丸中,31.1%在卵巢中) 显示出对立的甲基化变化,以应对父母和后代的温度治疗,表明补偿性表观遗传相互作用.
- 虽然具有累积温度影响的添加性DMRs很少见,但约5%的DMRs都是由温度引起的,并从亲子继承给后代,提供了多代表观遗传的证据.
- 现型数据显示,高温 (TT) 导致体重与对照人群相比较,性别比接近统计学意义,这表明表观遗传调节和现型可塑性之间存在联系.
结论:
- 温度显著塑造了欧洲海的表观基因组,影响了与性发育相关的DNA甲基化模式.
- 补偿性表观遗传相互作用在减轻温度压力的影响方面发挥作用,有助于表型可塑性.
- 环境敏感的多代表观遗传是一种可以促进物种适应不断变化的环境条件的机制,特别是在全球变暖的背景下.
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