内源激活多重DNA酶编码的纳米花用于长非编码RNA和癌症治疗的高对比成像
Huimin Yuan1, Zichen Jiao2, Tao Wang2
1School of Chemistry and Chemical Engineering, State Key Laboratory of Digital Medical Engineering, Southeast University, Nanjing 211189, China.
ACS nano
|October 9, 2025
概括
这项研究引入了新的ZnO封装DNA纳米花,用于精确的长非编码RNA (lncRNA) 检测和癌症治疗. 该纳米平台提供高对比度成像和在细胞和生物体中的向基因沉默.
科学领域:
- 生物医学工程 生物医学工程
- 分子生物学分子生物学
- 纳米技术 纳米技术
背景情况:
- 长非编码RNAs (lncRNAs) 在疾病和潜在的生物标志物中至关重要.
- 现有的DNA纳米材料面临信号泄漏和核酶不稳定等挑战.
- 滚动圆放大 (RCA) 产品具有潜力,但需要更好的细胞内集成.
研究的目的:
- 开发可酸激活的ZnO封装RCA纳米花,用于lncRNA成像和癌症治疗.
- 克服以前DNA纳米诊断和纳米治疗的局限性.
- 为了实现高对比的细胞内 lncRNA检测和向基因沉默.
主要方法:
- 设计的ZnO封装的RCA纳米花与DNA分裂DNA酶 (D-DNAzyme) 和RNA分裂DNA酶 (R-DNAzyme).
- 利用酸性瘤微环境进行ZnO分解,Zn2+释放和DNA酶激活.
- 使用R-DNAzyme切割基质探针,产生光和沉默幸存者基因.
主要成果:
- 在活细胞中实现了 lncRNA (HOTAIR) 的高对比成像,并在小鼠中进行实时监测.
- 经过酸刺激的脱离和核酶抵抗,减少背景信号.
- 成功区分了健康与癌症乳腺组织中的HOTAIR水平.
- 展示了R-DNA酶介导的幸存者基因淘汰和增强的光信号.
结论:
- 开发的纳米平台提供了准确的lncRNA测量和控制的癌症治疗.
- 酸可激活的ZnO-RCA纳米花提供了更好的成像对比度和治疗控制.
- 这种方法对诊断和治疗与lncRNA相关的疾病具有前景.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
02:34DNA Microarrays
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
02:39lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...