抗素定量ELISA测试在诊断刺激性肠综合征和炎症性肠病中的作用
Tran Khanh Chi1,2, Nguyen Thi Le My3, Hoang Hai Yen4
1Department of Biochemistry, Ha Noi Medical University, Ha Noi, Vietnam.
Medical archives (Sarajevo, Bosnia and Herzegovina)
|October 9, 2025
概括
反素自身抗体显示出诊断刺激性肠综合征 (IBS) 的潜力. 虽然该测试显示IBS的高特异性 (96.8%),但需要进一步的研究来改进其诊断模型,以区分IBS和炎症性肠病 (IBD).
科学领域:
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 温古林是一种细胞质蛋白质,在细胞粘附中起作用,并与调节肠道神经系统有关.
- 在患有刺激性肠综合征 (IBS) 的患者中观察到抗温林自身抗体的升高.
研究的目的:
- 建立和优化用于测量血清抗素抗体 (ATV) 水平的定量ELISA协议.
- 评估ATV测试对刺激性肠综合征 (IBS) 和炎症性肠病 (IBD) 的诊断效用.
- 评估ATV测试在区分IBS和IBD方面的潜力.
主要方法:
- 间接ELISA被开发和优化用于量化血清ATV.
- 在215名IBS患者,71名IBD患者和31名健康对照中测量了ATV度.
- 分析了ATV检测IBS的诊断性能及其区分IBS和IBD的能力.
主要成果:
- 优化的ATV ELISA显示出诊断IBS的潜力,曲线下的面积 (AUC) 为0.76 (p <0.001).
- 在510.59 ng/mL的截止值下,该测试实现了48.8%的IBS灵敏度和96.8%的特异性.
- 使用ATV水平来区分IBD和IBS的模型没有产生最佳结果.
结论:
- 定量ATV测试显示出作为IBS的诊断辅助的希望.
- 需要对更大,更统一的队列进行进一步的研究,以完善用于区分IBS和IBD的诊断模型.
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