Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Abnormal B-Cell Exosome Proapoptotic and Antiapoptotic Cargo in Multiple Sclerosis: Potential Implication in Progressive Disease Biology.

Neurology(R) neuroimmunology & neuroinflammation·2026
Same author

Cellular immunology data enable clinical severity prediction via supervised machine learning.

iScience·2026
Same author

PD-1 blockade drug holiday improves exhausted progenitor CD8 T cell (Tpex) reinvigoration by avoiding Tpex adaptive resistance.

bioRxiv : the preprint server for biology·2026
Same author

Perturbation of the preterm human immune system in early life.

JCI insight·2026
Same author

Single-cell profiling reveals diverse γδ T cell subsets in ulcerative colitis.

Science immunology·2026
Same author

Abnormal Pro-inflammatory Immune Cell Responses Precede Clinical Onset of Hypertensive Disorders of Pregnancy.

Hypertension (Dallas, Tex. : 1979)·2026
Same journal

CytoScan: Automated Detection of Technical Anomalies for Cytometry Quality Control.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

The 1st Mediterranean Meeting on Flow Cytometry: Forging New Collaborations Across the Mediterranean and Beyond.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Publication Guidelines for Optimized Multiparameter Immunolabeling Panels (OMIPs).

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

A Modular High-Parameter Flow Cytometry Framework: Pre-Analytical Optimization and Validation for Clinical Research.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Quantitative Detection of Entotic Cell-In-Cell Structures Using Deformable Segmentation and Deep Learning.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Comparison of Tissue Preparations to Identify and Phenotype T Cells in Human Colorectal Tumor Tissue.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
查看所有相关文章

相关实验视频

Updated: Jan 15, 2026

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry
06:51

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry

Published on: November 14, 2025

287

清洁网:基于蛋白质表达模式的细胞计数据中强大的双重检测.

Matei Ionita1, Michelle L McKeague1, Mark M Painter1,2

  • 1Institute for Immunology and Immune Health, University of Pennsylvania Perelman School of Medicine, Pennsylvania, USA.

Cytometry. Part A : the journal of the International Society for Analytical Cytology
|October 9, 2025
PubMed
概括
此摘要是机器生成的。

清洁网自动化了细胞计数据中的双重检测,改善了免疫细胞分析. 这种方法将单细胞与双细胞区分开来,使大规模研究和细胞相互作用的分析更准确.

更多相关视频

Counting Proteins in Single Cells with Addressable Droplet Microarrays
12:25

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

8.9K
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
08:40

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

13.4K

相关实验视频

Last Updated: Jan 15, 2026

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry
06:51

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry

Published on: November 14, 2025

287
Counting Proteins in Single Cells with Addressable Droplet Microarrays
12:25

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

8.9K
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
08:40

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

13.4K

科学领域:

  • 免疫学 免疫学 免疫学
  • 计算生物学 计算生物学
  • 生物技术是生物技术.

背景情况:

  • 使用流量和质细胞计的单细胞分析对于理解人类免疫学至关重要.
  • 分析大规模研究 (数百/数千个样本) 存在数据分析瓶.
  • 区分单细胞和双细胞 (多个细胞被检测为一个事件) 是一个关键的预处理步骤.

研究的目的:

  • 开发一种自动化方法,用于在细胞计数据中准确地检测和分类双重细胞.
  • 为了解决双重识别的传统双变门方法的局限性.
  • 为了使大型细胞计量数据集的更高效,更精确的分析.

主要方法:

  • 提出了Cleanet,这是一个由单细胞转录组学启发的新型自动化方法.
  • 清洁网模拟双重事件,并识别具有类似表达式配置文件的真实事件.
  • 在质量细胞计,流细胞计和成像流细胞计数据集上验证了该方法.

主要成果:

  • 在不同的细胞计量技术中,Cleanet准确地检测出同型和异型双胞胎.
  • 图像流细胞计证实,预测的双胞胎由多个细胞组成.
  • 证明Cleanet能够分类双重并检测细胞与细胞相互作用的能力,以治疗特异性的相互作用增加为例.

结论:

  • 清洁网提供了一种自动化,准确的解决方案,用于在细胞计量中进行双重检测和分类.
  • 该方法简化了大规模研究的数据分析,增强了免疫细胞分析.
  • 清洁网通过从丢弃的双重事件中提取信息来促进细胞与细胞相互作用的研究.