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Updated: Jan 15, 2026

One-step Purification of Twin-Strep-tagged Proteins and Their Complexes on Strep-Tactin Resin Cross-linked With Bissulfosuccinimidyl Suberate BS3
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One-step Purification of Twin-Strep-tagged Proteins and Their Complexes on Strep-Tactin Resin Cross-linked With Bissulfosuccinimidyl Suberate BS3

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通过静电辅助捕获改善Sortase-A介导的转化反应

Chen Wang1, Rémi Desmet2, Benoît Snella2

  • 1Centrale Lille, F-59000 Lille, France.

Organic letters
|October 9, 2025
PubMed
概括

研究人员通过添加多氨酸标签来设计Sortase A (SrtA) 酶基质. 这种修改通过捕获释放的来提高蛋白质修改产量,提高SrtA的效率.

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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