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TUBB1促进剂甲基化是预测慢性乙肝B的HBeAg血清转化的一个有前途的生物标志物

Tong Zhao¹, Yuna Tang¹, Yu Sun¹

¹Department of Hepatology, Qilu Hospital of Shandong University, Jinan, China.

Microbiology spectrum

|October 9, 2025

概括

在外周血液单核细胞 (PBMC) 中研究 TUBB1 促进体甲基化可以预测慢性乙型肝炎 (CHB) 患者的乙型肝炎e抗原血清转化 (HBeAg SC). 较低的基线TUBB1甲基化水平与成功的HBeAgSC相关,提供了一个新的预测生物标志物.

科学领域:

肝病学和免疫学肝病学和免疫学
表观遗传学和分子生物学
病毒性肝炎研究研究

背景情况:

乙型肝炎抗原血清转化 (HBeAg SC) 是慢性乙型肝炎 (CHB) 缓解的一个关键指标.
对HBeAg SC的预测生物标志物有限,阻碍了有效的患者监测.
在CHB进展中的DNA甲基化变化以前已经被注意到.

研究的目的:

调查TUBB1促进体甲基化在外周血液单核细胞 (PBMCs) 中作为CHB患者HBeAg SC的预测生物标志物的潜力.
确定在72周的随访期内,TUBB1促进体甲基化水平与HBeAg SC发生之间的关联.

主要方法:

招募了271名参与者:145名HBeAg阳性CHB,94名HBeAg阴性CHB和32名健康对照 (HC).
使用MethyLight和mRNA水平通过PBMCs的定量实时PCR量化TUBB1促进体甲基化的量化.
在72周内对HBeAg阳性CHB患者进行纵向随访,以评估HBeAg SC.

主要成果:

与HBeAg阴性CHB患者和HCs相比,在HBeAg阳性CHB患者中观察到增加的TUBB1促进剂甲基化和降低的TUBB1mRNA水平.
在不同的CHB阶段,TUBB1促进体甲基化水平下降,在获得HBeAg SC.的患者中,在基线时较低.
TUBB1促进物甲基化独立预测HBeAg SC (OR=0.683,P<0.001) 具有良好的预测值 (AUC=0.805,P<0.001).

结论:

在CHB患者中,TUBB1促进物甲基化显著改变,其水平随着疾病进展和血清转化而下降.
TUBB1促进物甲基化作为一个独立且可靠的非侵入性生物标志物,用于预测CHB患者的HBeAg SC.
这一发现为监测CHB和预测治疗结果提供了一个新的工具.

关键词:

在 HBeAg 血清转化过程中.一个管子,一个管子.慢性乙型肝炎慢性乙型肝炎促进物甲基化的促进物.

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