相关实验视频

Updated: Jan 15, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

ZmbZIP38与ZmDnaJ相互作用,通过调节ABA信号,口腔关闭和压力下的根生长来调节玉米苗的干旱耐受性

Anyi Dong¹, Nan Wang¹, Tinashe Zenda²

¹State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Crop Germplasm Resources in North China, Ministry of Education, College of Agronomy, Hebei Agricultural University, Baoding, Hebei, China.

Plant cell reports

|October 9, 2025

概括

转录因子ZmbZIP38通过与ZmDnaJ相互作用来增强玉米的干旱耐受性. 它调节了酸 (ABA) 生物合成,口腔关闭和根生长,改善了干旱压力下的植物生存.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

基本素拉链 (bZIP) 转录因子 (TF) 对植物干旱应激反应至关重要.
在调节玉米干旱耐受性方面,bZIP TFs的作用尚未完全被理解.
之前已经证明,ZmDnaJ通过abscisic acid (ABA) 生物合成和口腔关闭来增强玉米的干旱耐受性.

研究的目的:

确定和阐明ZmbZIP38作为ZmDnaJ在玉米干旱耐受性中的关键相互作用伙伴的功能.
调查玉米中ZmbZIP38介导干旱耐受性背后的分子机制.

主要方法:

酵母单杂交分析以确定ZmbZIP38作为ZmDnaJ的交互伙伴.
进行ZmDnaJ促进体分析以确定ZmbZIP38的直接向.
在玉米中进行基因过度表达和淘汰突变分析,以评估干旱耐受性表型.
生理和分子分析,包括ABA水平,ROS清理和基因表达.

主要成果:

ZmbZIP38通过在其推广者中绑定到ABRE动机,直接针对ZmDnaJ.
过度表达ZmbZIP38显著提高了干旱下玉米的存活率,增加了叶面积和口腔封闭.
过度表达ZmbZIP38的线条显示出改善的ROS清理,减少氧化损伤,以及由于调节ABA生物合成基因而增加的ABA水平.
zmbzip38的淘汰突变体表现出干旱耐受性降低,细胞损伤增加,叶面积减少.

结论:

ZmbZIP38与ZmDnaJ相互作用,增强玉米的干旱耐受性.
在干旱压力下,ZmbZIP38调节ABA生物合成和信号,口腔关闭,ROS清理和根生长.
ZmbZIP38是玉米苗木干旱耐受性的关键调节剂,具有显著的潜力,可以改善作物对非生物应激的抵抗力.

关键词:

阿巴阿巴阿巴是什么意思耐旱耐受性耐旱耐受性耐旱耐受性玉米玉米玉米是一种这就是ZmbZIP38的ZmbZIP38 bZIP转录因子 bZIP转录因子

更多相关视频

Imaging and Analysis for Quantifying Maize (Zea mays) Abiotic Stress Phenotypes

Imaging and Analysis for Quantifying Maize (Zea mays) Abiotic Stress Phenotypes

Published on: March 28, 2025

Kinematic Analysis of Cell Division and Expansion: Quantifying the Cellular Basis of Growth and Sampling Developmental Zones in Zea mays Leaves

Kinematic Analysis of Cell Division and Expansion: Quantifying the Cellular Basis of Growth and Sampling Developmental Zones in Zea mays Leaves

Published on: December 2, 2016

相关概念视频

Adaptations that Reduce Water Loss

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.

Regulation of Transpiration by Stomata

Regulation of Transpiration by Stomata

During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.

Responses to Drought and Flooding

Responses to Drought and Flooding

Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.