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Updated: Jul 13, 2026

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model
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用于测量粘附细胞中 DED 纤维的 caspase-8 活性的协议

Corinna König1, Inna N Lavrik1

  • 1Translational Inflammation Research, Medical Faculty, Center of Dynamic Systems, Otto von Guericke University, Magdeburg, Germany.

STAR protocols
|October 9, 2025
PubMed
概括

这项研究引入了一种方法来测量死亡诱导信号复合体 (DISC) 中的酶-8活性. 该协议允许在其原生复合体内对亡研究中的caspase-8激活进行直接分析.

关键词:
癌症 癌症 癌症 癌症细胞生物学 细胞生物学基于细胞的测试

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相关概念视频

Caspases01:24

Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside cells.