工程 Tetrahydrodaidzein 减少酶以消除逆催化,以实现 (S) -Equol 的高效生物合成
Tianmeng Zhang1,2,3, Qihang Chen1,2, Yunpeng Liu1,2,3
1State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China.
Journal of agricultural and food chemistry
|October 9, 2025
概括
研究人员设计了一种关键的酶,四化酶还原酶 (THDR),以改善 (S) -equol的产生,这是一种健康食品和化品中发现的有益化合物. 这种增强的酶通过微生物发酵显著增加了 (S-equol) 的产量.
科学领域:
- 生物催化和酶工程 生物催化和酶工程
- 代谢工程是代谢工程.
- 功能性食品的成分 功能性食品的成分
背景情况:
- (S) -Equol是一种有价值的异黄代谢物,用于健康食品,补充剂和化品.
- 甲基二氧化二氧化还原酶 (THDR) 对于将二氧化转化为 (S) -equol至关重要,但其效率较低.
- 了解THDR的催化机制是提高其性能的关键.
研究的目的:
- 通过量子化学阐明四二氧化二氧化还原酶 (THDR) 的反应机制.
- 设计THDR以提高 (S) -equol生产效率.
- 为 (S) -equol.建立一个可扩展的微生物发酵过程.
主要方法:
- 量子化学集群模型计算,以调查THDR催化机制.
- 通过重新设计基板,催化和水通道来创建THDR的蛋白质工程,以创建M3-THDR变体.
- 在5L生物反应器中优化微生物发酵.
主要成果:
- 该研究阐明了THDR涉及FADH2.2的可逆催化机制.
- 工程设计的M3-THDR变体显示出高的前向脱氧化效率和抑制的反向氧化.
- 通过微生物发酵,M3-THDR通过微生物发酵实现了创纪录的4700 mg/L (S) -equol产量.
结论:
- 机械引导的THDR工程显著提高了 (S) -equol生产.
- 该M3-THDR变种代表了在实现工业应用的高产量的突破.
- 这项研究为通过酶优化扩大功能成分生产提供了一个框架.
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