在2-6°C保存5天的解新鲜冷血的凝血因子稳定性和无菌性:为了优化利用率
Ruchika Bhartia1, Paramjit Kaur1, Kshitija Mittal1
1Government Medical College and Hospital, Chandigarh, India.
Hematology, transfusion and cell therapy
|October 9, 2025
概括
解的新鲜冷血在2-6°C下维持了5天的凝血因子水平和无菌性. 这表明,对于创伤患者来说,这种关键血液产品的保质期可能会延长.
科学领域:
- 输血医学 输血医学
- 血液学 血液学 血液学
- 临床病理学 临床病理学
背景情况:
- 新鲜冷血 (FFP) 对于治疗出血和创伤患者至关重要.
- 由于担心不稳定凝血因子的下降,限制FFP的使用时间超过24小时.
- 这项研究调查了长期冷藏后的FPF质量.
研究的目的:
- 评估在2-6°C下存储5天的解FP中的凝结因子水平.
- 为了评估长期储存期间FPF的微生物污染.
- 确定延长FPF保质期的可行性.
主要方法:
- 对40名男性献血者 (A和O血型) 进行前性观察研究.
- 在解后的5天内,FFP位在2-6°C下存储.
- 测量了凝血测定 (PT,aPTT,INR) 和因子活性 (V,VIII,IX,纤维素,vWF). 无菌性测试是在第5天进行的.
主要成果:
- 在储存期间观察到PT,aPTT和INR的显著增加 (p < 0.001).
- 第五因子和第八因子活动显著下降 (分别为20.0%和42.2%,p<0.001),大部分第八因子在前24小时下降.
- 在O组血中,·威莱布兰德因子活性较低 (p < 0.05);所有培养物保持无菌.
结论:
- 关键的凝固因子在解的FFP中保持在2-6°C的温度下5天.
- 产品无菌性在整个五天的储存期内保持.
- 在2-6°C的温度下延长化FP的储存是一个可行的选择,可能会增加其实用性.
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