Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

8.7K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
8.7K
Protein Modifications in the RER01:26

Protein Modifications in the RER

6.9K
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
6.9K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

13.7K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
13.7K
Translational Regulation01:29

Translational Regulation

533
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
533
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.3K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.3K
Bacterial Protein Maturation01:26

Bacterial Protein Maturation

460
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
460

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Depletion-Free Automated Enrichment of Serum Glycopeptides for High-Throughput Clinical Glycoproteomics.

Molecular & cellular proteomics : MCP·2026
Same author

RPS6KA3/RSK2-mediated phosphorylation of DRAM2 promotes lysosomal targeting and autophagic flux in melanoma.

Autophagy·2026
Same author

StrucPTM: a database of structurally validated protein modifications and their conformational variation.

Bioinformatics (Oxford, England)·2026
Same author

Non-Reducing Proteomics Reveals Disulfide-dependent Proteoform Remodeling Under Oxidative Stress.

Molecular & cellular proteomics : MCP·2026
Same author

Assessing Long-Term Stored Tissues for Multi-Omics Data Quality and Proteogenomics Suitability.

Journal of proteome research·2025
Same author

Hidden route of protein damage through oxygen-confined photooxidation.

Nature communications·2024

相关实验视频

Updated: Jan 15, 2026

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
09:10

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

9.9K

SAPP:结构意识到PTM预测

Yujin Choo1, Seungjin Na2, Eunok Paek3

  • 1Department of Artificial Intelligence, Hanyang University, Seoul, 04763, Republic of Korea.

Computers in biology and medicine
|October 9, 2025
PubMed
概括

我们开发了SAPP,这是一个新的结构意识模型,用于预测翻译后修改站点. 通过将蛋白质结构与序列数据集成,SAPP提高了细胞机制的预测准确性和生物相关性.

关键词:
深度学习是一种深度学习.后翻译修改后的修改.蛋白质结构 蛋白质结构转移学习转移学习变压器变压器变压器

更多相关视频

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function
05:57

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function

Published on: April 26, 2024

833
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.3K

相关实验视频

Last Updated: Jan 15, 2026

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
09:10

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

9.9K
Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function
05:57

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function

Published on: April 26, 2024

833
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.3K

科学领域:

  • 生物化学 生化学
  • 计算生物学 计算生物学
  • 结构生物学 结构生物学

背景情况:

  • 翻译后修饰 (PTMs) 对于调节各种细胞过程至关重要,包括信号传导,细胞生长和分化.
  • 准确识别PTM部位对于理解细胞机制和开发向疗法的必要.
  • 现有的计算模型经常忽视结构上下文,如内在无序区域和溶剂可访问性,限制预测准确性.

研究的目的:

  • 引入SAPP (结构意识的PTM预测),这是一个用于PTM站点预测的新计算框架.
  • 将蛋白质结构信息与序列数据集成,以改善预测.
  • 证明基于结构的模型在PTM预测中的实用性,包括对有限数据的PTM类型的概括.

主要方法:

  • 开发了SAPP,这是一个基于变压器的模型,利用自我注意和交叉注意机制.
  • 综合结构特征来自AlphaFold2预测与序列信息.
  • 使用酸化预训练模型对其他PTM类型进行微调.

主要成果:

  • SAPP有效地捕捉了蛋白质序列及其结构状态之间的复杂相互作用.
  • 与仅依据序列的模型相比,结构意识的方法显著提高了PTM预测准确性和生物相关性.
  • 对于具有有限培训数据的PTM类型,SAPP表现出强大的泛化性能.

结论:

  • 结构信息对于准确的PTM站点预测至关重要.
  • SAPP代表了基于结构的PTM预测的开创性框架,推动了该领域的发展.
  • 这项工作加深了对PTMs生物意义的理解,并为治疗开发开辟了道路.