一种新型的菌素lysin Lys19可以作为潜在的生物控制剂对抗Clostridium perfringens和Streptococcus suis
Yalu Ji1, Yuqing Zhou1, Yao Fu1
1State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, 130062, China.
International journal of food microbiology
|October 9, 2025
概括
一种新型的菌素lysin,Lys19,有效地准了Clostridium perfringens和多种Streptococcus suis血清型. 这一发现为减少肉类和农产品中食物传播病原体提供了一个有希望的新策略.
科学领域:
- 微生物学 微生物学
- 食品安全 食品安全
- 生物技术是生物技术.
背景情况:
- 食品受到Clostridium perfringens (C. perfringens) 和Streptococcus suis (S. suis) 的污染对人类健康构成重大风险.
- 迫切需要有效的战略来缓解食品中的微生物污染.
研究的目的:
- 为了分离和表征一种新型细菌菌素lysin,Lys19,来自C. perfringens菌 XBG01.01衍生.
- 评估Lys19对C. perfringens和S. suis的性活性,稳定性和抗菌膜疗效.
主要方法:
- 对C. perfringens菌体XBG01及其素Lys19.1的分离和表征.
- 在Lys19.9的催化域内确定关键活性位点.
- 评估Lys19在不同温度,pH值和不同细菌血清型中的性活性.
- 评估Lys19在预防和消除C. perfringens生物膜上的有效性,并减少生猪肉中的细菌负载.
主要成果:
- 莱斯19对类型A C. perfringens和多种S. suis血清型表现出强烈的性活性,显示出跨物种的有效性.
- 素在广泛的温度范围 (4-55°C) 和pH范围 (3-9) 中保持稳定的活性.
- 莱斯19有效地预防和消除了C. perfringens生物膜,显著降低了生菜 (88.75%的降低) 和生猪肉 (84.65%的降低) 上的细菌活力.
结论:
- 莱斯19是第一个报告的C. perfringens菌素,具有跨细菌物种的解毒功能.
- 莱斯19表现出强大的稳定性和强大的抗菌膜和杀菌活性,对抗C. perfringens和S. suis.
- 莱斯19在控制肉类和农产品中的食源性病原体方面具有显著的应用潜力,提高了食品安全.
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