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相关概念视频

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

1.4K
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
1.4K
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

754
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
754
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

616
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
616
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

1.9K
Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
1.9K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.5K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.5K
¹H NMR: Long-Range Coupling01:27

¹H NMR: Long-Range Coupling

2.6K
The coupling interactions of nuclei across four or more bonds are usually weak, with J values less than 1 Hz. While these are usually not observed in spectra, the presence of multiple bonds along the coupling pathway can result in observable long-range coupling.
In alkenes, spin information is communicated via σ–π overlap, as seen in allylic (four-bond) and homoallylic (five-bond) couplings. These coupling interactions are stronger when the σ bond is parallel to the alkene...
2.6K

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相关实验视频

Updated: Jan 15, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
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使用T-HMQC序列,在两个不同的半整数四极核之间进行透过空间的NMR相关性.

Yury G Kolyagin1, Julien Trébosc2, Olivier Lafon1

  • 1Univ. Lille, CNRS, Centrale Lille, Univ. Artois, UMR 8181, UCCS - Unité de Catalyse et de Chimie du Solide, F-59000, Lille, France.

Solid state nuclear magnetic resonance
|October 9, 2025
PubMed
概括

这项研究介绍了T-HMQC,这是一种用于探测四极核之间的近距离的新型NMR技术. T-HMQC提供更广泛的激发带宽,有利于分析像眼镜这样的复杂材料.

关键词:
D-HMQCCC 的意思这是四极核.在T-HMQCC.捕者是什么意思通过空间的相关性.

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Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
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科学领域:

  • 固态核磁共振 (NMR) 光谱学. 固态核磁共振 (NMR) 光谱学.
  • 材料科学,特别是玻璃和无形材料的研究.

背景情况:

  • 异质核多量子相关性 (HMQC) 实验用于研究旋转-1/2核之间的近距离.
  • 人口转移在双共振 (TRAPDOR) 实验中使用特定的辐射技术.

研究的目的:

  • 为了证明T-HMQC的适用性,探测不同的半整数旋转四极同位素之间的近距离.
  • 引入T-HMQC的新型选择性变体,以减少光谱复杂性.
  • 分析实验参数对T-HMQC效率的影响.

主要方法:

  • 开发和应用T-HMQC (TRAPDOR-HMQC) 序列用于四极核.
  • 引入选择性T-HMQC变体,使用回声-反回声二次检测或中央过渡 (CT) 选择性脉冲.
  • 在氨酸玻璃上对B-27Al T-HMQC进行实验分析.

主要成果:

  • T-HMQC有效地探测半整数旋转四极同位素之间的近距离.
  • 优化的TRAPDOR脉冲条件涉及整数转子周期和最大rf场强度的分离.
  • 选择性T-HMQC变体减少了光谱复杂性,但与D-HMQC相比,可能会降低灵敏度.

结论:

  • T-HMQC是一种有价值的技术,用于研究材料中的四极核相互作用.
  • 对于高效的T-HMQC,TRAPDOR脉冲同步和rf振幅至关重要.
  • T-HMQC提供了更宽的激发带宽,有利于广泛的NMR光谱,特别是在高场.