解读了来自三个不同医院未经处理的废水中的流动性,致病宿主和抗生素耐药基因的共同选择
Yanying Feng1, Qiheng Yuan2, Longji Wang3
1Department of Internal Medicine, The First Clinical Medical College, Shanxi Medical University, Taiyuan, Shanxi Province, China.
概括
医院的废水含有众多抗生素耐药性基因 (ARG),可以通过移动遗传元素和与金属/杀菌剂耐药性共同选择传播. 迫切需要废水处理,以减轻公共卫生风险.
科学领域:
- 环境微生物学环境微生物学
- 公共卫生 公共卫生
- 基因组学就是基因组学.
背景情况:
- 医院的废水是抗生素耐药性基因 (ARG) 的重要来源.
- 涉及金属/杀菌剂耐药性基因 (MRGs/BRGs) 和移动遗传元素 (MGE) 的共同选择机制尚不清楚.
- 了解这些因素对于公共卫生和环境安全至关重要.
研究的目的:
- 综合评估医院废水中的ARG,MRG和BRG.
- 研究它们的丰富性,移动性,病原性宿主和共同选择模式.
- 在不同类型的医院中评估与这些耐药基因相关的风险.
主要方法:
- 来自三种医院类型的未经处理的废水的元基因组测序和组装.
- 使用SARG和BacMet数据库识别ARG,MRG和BRG.使用SARG和BacMet数据库识别ARG,MRG和BRG.
- 使用PlasFlow和MOB套件对ARG宿主,移动性和MGE同时发生的分析.
主要成果:
- 检测到1911个ARG,1662个MRG和916个BRG,其中四环素,多种药物和β-乳糖抗性基因占主导地位.
- 发现了ARG的显著等离子体关联 (46.43%),并确定了关键病原体如Klebsiella pneumoniae和Enterococcus spp.中的高风险ARG.
- 观察到ARG与MGE和MRG/BRG,特别是高风险ARG (76.2%) 在传统中医医院的废水中大量同时发生.
结论:
- 未经处理的医院废水是ARG的主要储存库,由于致病宿主,MGE介导的水平基因转移和共同选择,造成了重大风险.
- 迫切需要有效的废水处理策略来控制抗生素耐药性的传播.
- 结果为未来的干预措施提供信息,以打击医疗保健机构中的抗微生物药物耐药性.
相关概念视频
Antibiotic Selection
59.4K
Overview
59.4K
Development of Antibiotic Resistance
1.3K
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
1.3K
Gene Regulation in Microbial Communities: Quorum Sensing
518
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
518
Infection
11.8K
When a pathogen enters the body and reproduces, it can cause an infection, damage body cells, and cause illness symptoms that eventually lead to disease. Therefore, its prevention requires breaking the chain of infection.
The chain begins with pathogens: bacteria, viruses, fungi, prions, or parasites such as protozoa helminths. These can be present on the skin as transient or resident flora, or they can be acquired from the environment. Identifying and treating the type of infection and...
The chain begins with pathogens: bacteria, viruses, fungi, prions, or parasites such as protozoa helminths. These can be present on the skin as transient or resident flora, or they can be acquired from the environment. Identifying and treating the type of infection and...
11.8K


