相关实验视频

Updated: Jan 15, 2026

Measurements of Physiological Stress Responses in C. Elegans

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

在小鼠中注射卡因酸盐后,展开蛋白质反应的区域和细胞类型特定激活

Ly Huong Nguyen¹, Loc Dinh Nguyen¹, Dat Xuan Dao¹

¹Department of Neuroanatomy, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Ishikawa, Japan.

Neurochemistry international

|October 9, 2025

概括

在刺激毒性后,展开的蛋白质反应 (UPR) 在大脑中被激活. 特定神经元中的UPR激活与神经元活动和存活相关,这表明神经保护作用.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

展开的蛋白质反应 (UPR) 在细胞应激反应中至关重要.
在像脑缺血和神经退行等神经病理中观察到UPR激活.
激活转录因子6 (ATF6) 和内细胞网膜 (ER) 伴侣体显示神经保护对刺激毒性.

研究的目的:

研究在小鼠大脑中刺激性损伤后UPR激活的时间和空间动态.
探索UPR激活,神经元活动和神经元存活之间的关系,以应对kainat (KA) 诱导的兴奋毒性.

主要方法:

利用RT-qPCR量化UPR基因表达变化后的内囊KA注射.
使用ER压力激活指标 (ERAI) -转基因小鼠通过GFP信号可视化UPR激活 (拼接XBP-1).
在特定的神经元群体 (CA3,牙状,CA1) 和星球细胞中分析了UPR局部化.

主要成果:

UPR基因表达显著增加,在KA给药后1或3天达到峰值.
在CA3神经元中,ERAI衍生的GFP信号突出,在牙状环神经元中中度,但在CA1神经元中不存在.
在高神经元活动和抗KA毒性区域的神经元 soma和树突中检测到ERAI信号,其中包括一些天体细胞参与.

结论:

这项研究表明,在刺激毒性攻击后,海马体内有动态的UPR激活模式.
UPR激活与神经元活动和抵抗KA诱导的兴奋毒性的空间相关.
这些发现表明UPR在神经元活动和生存的背景下具有潜在的神经保护作用.

关键词:

激发性毒性激发性毒性蛋白质稳定性蛋白质稳定性不折叠的蛋白质反应反应

更多相关视频

Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic

Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic

Published on: September 4, 2012

Urokinase-type Plasminogen Activator-induced Mouse Back Pain Model

Urokinase-type Plasminogen Activator-induced Mouse Back Pain Model

Published on: September 1, 2023

相关概念视频

The Unfolded Protein Response

The Unfolded Protein Response

The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...

Regulation of the Unfolded Protein Response

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...