外源H2S调节生长,在盐应激下以度依赖的方式在番茄苗中调节生长和抗氧化防御
Rewaj Subba1,2, Soumya Mukherjee3, Piyush Mathur4,5
1Microbiology Laboratory, Department of Botany, University of North Bengal, Raja Rammohunpur, Dist. Darjeeling, West Bengal, 734013, India.
Protoplasma
|October 9, 2025
概括
外源硫化 (H2S) 通过减少氧化损伤和增强抗氧化防御,减轻番茄苗的盐应激. 这种气体递质在中度至高度的NaCl下改善生长,这表明它在植物应激反应中的关键作用.
科学领域:
- 植物生理学 植物生理学
- 生物化学 生物化学
- 环境压力生物学
背景情况:
- 硫化 (H2S) 作为一种气体传递物,对植物生长,发育和承受压力至关重要.
- 盐度压力对作物产量和番茄等植物的代谢过程产生负面影响.
研究的目的:
- 为了研究外源硫化 (H2S) 在不同NaCl应激水平下对番茄幼苗的代谢特征的影响.
- 确定H2S对盐引起的氧化损伤的保护作用及其对关键生理参数的影响.
主要方法:
- 番茄幼苗接受了不同度的NaCl (0-120mM) 和外源H2S捐赠者 (NaHS在0,25,100μM).
- 测量包括MDA含量,电解质泄漏,Na+/K+比,林,叶绿素,RWC,内源H2S,L-DES活性,酸盐和抗氧化酶活性 (GR,CAT,APOD,POD,SOD).
主要成果:
- 外源H2S的应用降低了MDA含量,电解质泄漏,Na+/K+比率,并在NaCl应激下降低了proline.
- 在80毫米的NaCl,H2S (100微米) 增加了叶绿素,RWC,内源的H2S,L-DES活性和酸盐含量.
- 在H2S处理中,关键的抗氧化酶 (包括GR,CAT,APOD,POD和SOD) 的高调,减轻了盐引起的氧化应激.
结论:
- 外源H2S,特别是在100μM,显著减轻了在80mM NaCl.以下种植的番茄苗的盐应激.
- H2S信号传递在调解长距离对NaCl应激反应方面发挥着至关重要的作用,通过依赖度的机制增强了植物的耐受性.
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