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与阿米巴的相互作用驱动了Candida haemulonii复合体中毒性相关的表型

Artid Amsri^1,2, Kritsada Pruksaphon^3,4, Patcharin Thammasit²

¹Office of Research Administration, Chiang Mai University, Chiang Mai, Thailand.

|October 9, 2025

概括

与Acanthamoeba castellanii的相互作用可以增强Candida duobushaemulonii的毒性. 阿米巴的适应促进了表型变化,增加了生物膜的形成和毒性基因表达,从而导致更大的致病性.

科学领域:

微生物学微生物学
菌类学菌类学是指菌类学.
病变的发生和发病.

背景情况:

阿米巴的捕食可以诱导微生物的适应性特征,可能增强它们的弹性和病原性能力.
环境真菌Candida haemulonii复杂物种越来越多地因其在机会性感染中的作用而得到认可.

研究的目的:

调查与Acanthamoeba castellanii的相互作用是否调节环境中的Candida haemulonii复杂物种的毒性.
为了确定C. duobushaemulonii在适应菌宿主后的表型和遗传变化.

主要方法:

将C. duobushaemulonii与Acanthamoeba castellanii进行培养,以诱导适应.
适应和非适应分离物的表型特征,包括聚合,丝和生物膜形成试验.
病毒性相关和生物膜相关基因的转录分析.
酶活性测定 (雌激酶,脂酶,分泌的阿斯巴提尔蛋白酶).
在Galleria mellonella中进行致病性测定.

主要成果:

在C. duobushaemulonii中,阿米巴的适应诱导了四种不同的表型,并增加了聚合和细分.
适应的分离体显示显著更高的生物膜生物质 (超过15倍) 和细胞外基质生产 (超过2.2倍).
观察到关键毒性基因 (例如,WOR1,CPH1,HGC1) 和生物膜基因 (例如,ALS1,BCR1,EGF1) 的升级.
他们注意到对环境压力因素的耐受性提高和水解酶的分泌量增加.
致病性测定表明,适应的隔离物显著增加了Galleria mellonella的毒性,致命剂量大幅降低 (LD50).

结论:

环境与虫的相互作用促进了C. duobushaemulonii中毒性相关表型的出现.
阿米巴的适应可以作为理解环境压力如何促进真菌病原的模型.
这些发现强调了宿主-病原体相互作用在塑造微生物毒性和适应中的作用.

关键词:

这是Candida haemulonii.梅罗内拉画廊 (Galleria mellonella) 是一个著名的画廊.抗真菌易感性抗真菌易感性土壤阿米巴土壤阿米巴毒性因子是毒性因子.

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