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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Updated: Jan 15, 2026

NanoDrop Microvolume Quantitation of Nucleic Acids

NanoDrop Microvolume Quantitation of Nucleic Acids

Published on: November 22, 2010

通过DNA驱动的纳米化学定位量化纳米粒子.

Tong Xia¹, Ziyue Guo¹, Lei Song¹

¹Hefei National Research Center for Physical Sciences at the Microscale, Center for Bioanalytical Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui 230026, China.

|October 9, 2025

概括

此摘要是机器生成的。

研究人员开发了一种新的定位方法来测量纳米颗粒分子度. 这种技术使用DNA可编程组件进行准确的量化,简化了纳米粒子的表征.

关键词:

这是一个DNA可编程组件.化学定位定位化学定位定位摩拉灭绝系数摩拉灭绝系数纳米颗粒的二极管.纳米颗粒量化的量化方法

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Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer

Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer

Published on: February 17, 2011

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Last Updated: Jan 15, 2026

NanoDrop Microvolume Quantitation of Nucleic Acids

NanoDrop Microvolume Quantitation of Nucleic Acids

Published on: November 22, 2010

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer

Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer

Published on: February 17, 2011

科学领域:

纳米技术纳米技术
分析化学分析化学
生物化学生化学

背景情况:

纳米合成提供了各种各样的纳米材料,具有先进的功能.
精确的摩尔度测量复杂的纳米粒子仍然是一个挑战.

研究的目的:

开发一种用于确定纳米粒子分子灭绝系数的通用化学方法.
为了能够在没有先前的组成或形态数据的情况下准确量化纳米粒子.

主要方法:

利用DNA可编程组件来驱动纳米粒子相互作用.
在标定剂和分析剂纳米粒子之间形成1:1的固态度异构体.
运用光谱解卷法来计算分子灭绝系数.

主要成果:

成功确定了各种纳米粒子的分子灭绝系数.
该方法证明了对纳米粒子损失 (吸附/沉) 的稳定性.
建立了纳米粒子分子灭绝系数的统一标准,使用金纳米球作为常见标称.

结论:

开发的基于定位的方法提供了一种可靠的方法来量化纳米粒子分子度.
这种方法简化了纳米粒子的表征,并促进了实验室间的比较.
该技术克服了复杂纳米材料现有方法的局限性.