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在pterygium中不同表达的LncRNAs配置文件

Jiusheng Zheng¹, Zhixin Ding², Lixin Mei³

¹Department of Ophthalmology, The First People's Hospital of Changzhou, Changzhou, Jiangsu Province, China.

BMC ophthalmology

|October 9, 2025

概括

长非编码RNAs (lncRNAs) 在pterygium组织中具有差异性表达. 关键的 lncRNAs HOTTIP 和 RP1-261G23.7 显示出作为菌体的新型治疗点的潜力.

科学领域:

眼科医生眼科眼科
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

是常见的眼睛表面疾病.
长非编码RNAs (lncRNAs) 在pterygium病原发生中的作用尚未完全理解.

研究的目的:

通过基因芯片技术在pterygium中识别差异表达的lncRNAs.
研究特定的lncRNAs,HOTTIP和RP1-261G23.7在pterygium发育中的潜在机制.

主要方法:

基因芯片技术被用来选20个质组织和20个正常结膜组织中的lncRNA表达.
使用定量实时聚合酶链反应 (qRT-PCR) 来验证选定lncRNAs (HOTTIP,RP1-261G23.7) 和它们的基因 (HOXA13,VEGFA) 的表达.

主要成果:

在pterygium和正常结膜组织之间共鉴定了8271个差异表达的lncRNAs.
在pterygium组织中,有612个lncRNAs被上调和743个被下调.
发现 lncRNAs HOTTIP,RP1-261G23.7,以及它们的基因HOXA13和VEGFA的表达水平高度一致,并在对照组表达.

结论:

长非编码RNAs在pterygium中具有差异性表达.
的 lncRNAs HOTTIP 和 RP1-261G23.7 是潜在的新型基因点对的治疗.

关键词:

长非编码RNA (lncRNA) 是一种长非编码RNA.作用机制作用机制在Pterygium的基础上.定量实时聚合酶连锁反应 (qRT-PCR) 是一种目标基因是目标基因.

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相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...