将淋巴血管系统整合到人体淋巴结上芯片中可以增强生理免疫特性
Andrew I Morrison1,2, Jonas Jäger1,2, Charlotte M de Winde1,2,3
1Amsterdam UMC Location Vrije Universiteit Amsterdam, Molecular Cell Biology & Immunology, De Boelelaan 1117, Amsterdam, the Netherlands.
Materials today. Bio
|October 10, 2025
概括
研究人员开发了一种新的器官在芯片上的淋巴结模型,其中集成了淋巴血管. 这种模型增强了免疫细胞的功能和迁移,为研究免疫反应和开发疗法提供了新的途径.
科学领域:
- 免疫学 免疫学 免疫学
- 生物工程是生物工程.
- 微生理系统 微生理系统
背景情况:
- 研究人类免疫反应需要先进的体外模型来模拟淋巴结微环境.
- 淋巴结 (LN) 的复杂性,包括淋巴血管,对微生理系统 (MPS) 构成挑战.
研究的目的:
- 在一个带有整合淋巴系统的器官芯片 (OoC) 平台上建立一个有机型的淋巴结模型.
- 为了研究透的淋巴内皮细胞 (LEC) 线通道对LN-on-chip微环境的影响.
主要方法:
- 开发了一个有机型的LN模型,使用一个OoC平台和一个打印的牺牲结构.
- 在LN模型中集成了一个透道,里面着LECs.
- 在淋巴流下培养模型一周,以评估LEC行为和微环境稳定性.
主要成果:
- 在LN模型中成功形成了由LEC嵌的功能性淋巴血管.
- 确认了稳定的代谢条件和LN特定的LEC标记物的上调 (例如,ACKR4).
- 观察到免疫细胞 (特别是B细胞) 的增加,增强IL-7和CCL21的分泌,并促进免疫细胞在血管附近聚集.
结论:
- 开发的LN-on-chip模型与集成的淋巴系统为研究免疫细胞迁移和相互作用提供了增强的生理微环境.
- 这个平台促进了对免疫交叉和自适应性免疫反应的研究.
- 该方法可以集成到多器官芯片系统中,进行全面的免疫学研究.
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