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在食发酵中消化的料的新陈代谢概况,通过各种溶剂提取分化
Ayu Septi Anggraeni1, Anuraga Jayanegara2, Anjar Windarsih1
1Research Center for Food Technology and Processing, National Research and Innovation Agency, DI Yogyakarta, Indonesia.
Journal of advanced veterinary and animal research
|October 10, 2025
概括
确定了分析总混合配给 (TMR) 料和牛体液代谢物质的最佳溶剂条件. 100%的甲醇有效地区分了TMR料,而50%的甲醇和水混合物则在反液分析中表现出色.
科学领域:
- 代谢学 代谢学 代谢学
- 分析化学 分析化学
- 动物营养 动物营养
背景情况:
- 准确的动物料和消化样本的代谢分析对于了解动物健康和营养至关重要.
- 在料中加入基托桑可以改变样本矩阵,需要优化分析方法.
- 超高性能液态色谱-高分辨率质谱 (UHPLC-HRMS) 是一种用于代谢分析的强大工具.
研究的目的:
- 为了确定最佳的溶剂条件,对总混合配给 (TMR) 料和反动物液代谢组进行敏感,特异和可重复的分析.
- 评估基托桑添加对样品制备和代谢物检测的影响.
- 为了确定TMR料和反体液的关键代谢物标志物.
主要方法:
- 使用三种溶剂比率制备了含有或不含0.5%奇托桑的TMR料和反液样本:50%水:50%甲醇,20%水:80%甲醇和100%甲醇.
- 使用超高性能液态染色学高分辨率质谱法 (UHPLC-HRMS) 进行了代谢组分析.
- 使用统计分析来评估样本歧视,并确定重要的代谢物标记物.
主要成果:
- TMR料样本产生了311种代谢物,主要是氨基酸,脂肪酸和氨基.
- 液样本含有39种代谢物,其中脂肪酸,有机酸和氨酸占主导地位.
- 100%的甲醇为TMR料样本提供了最佳的区分,突出显示了酸,D-(+) - 烯和L(+) - 甲素作为关键标记.
- 50%的甲醇-水溶剂比率实现了小液样本的最佳分辨率,三乙烯酸盐,二胺和酸被确定为关键标记物.
结论:
- 对于TMR料代谢分析的最佳溶剂是100%的甲醇.
- 50%的甲醇-水溶剂混合物是牛体液代谢分析的最佳选择.
- 这些发现使得动物营养研究中更准确,更可靠的代谢学研究成为可能.
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