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Masaki Yamamoto¹, Michael T White², Takahisa Kawaguchi³

¹Department of Clinical Laboratory Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

Frontiers in microbiology

|October 10, 2025

概括

诊断过去的COVID-19感染是具有挑战性的. 将T-SPOT测试与抗体测试相结合,可显著改善先前的SARS-CoV-2感染的检测,有助于流行研究.

科学领域:

免疫学免疫学免疫学
传染性疾病传染性疾病
诊断检测试验诊断检测试验

背景情况:

准确诊断之前的COVID-19感染仍然是一个临床挑战.
评估过去的SARS-CoV-2感染对于流行病学研究和了解人口免疫力至关重要.

研究的目的:

评估T-SPOT测试的疗效,结合抗体测试,用于诊断之前的SARS-CoV-2感染.
评估冷的外周血液单核细胞 (PBMC) 对T-SPOT测定的有用性.

主要方法:

利用T-SPOT测定 (向核囊和膜蛋白) 和抗体测试 (向核囊蛋白) 在122例PCR确诊的COVID-19病例和67例对照组中.
通过T-SPOT发现SARS-CoV-2试验和通过iFlash-SARS-CoV-2 IgG和MAGPIX试验的幽默免疫分析了细胞介导免疫反应.
使用灵敏度,特异性和接收器操作特征曲线 (AUC) 下的面积来评估诊断性能.

主要成果:

抗体测试显示了类似的敏感性 (64.8%-67.2%) 和高特异性 (>98.4%).
T-SPOT.N测试显示了与抗体测试相似的性能 (灵敏度:62.5%,特异性:98.4%,AUC:0.923).
与MAGPIX抗体测试结合T-SPOT.N试验,与单个试验相比,显著提高了灵敏度 (88.0%) 和AUC (0.979).

结论:

冷的PBMC适用于T-SPOT测定性能.
结合T-SPOT测定和抗体测试,可以更好地诊断过去的SARS-CoV-2感染,特别是在接种疫苗的人群中.
整合细胞和幽默免疫测试为COVID-19流行研究提供了一个有希望的方法.

关键词:

在 COVID-19 疫情中,伊格拉 (IGRA) 是一个这就是T-SPOT.抗体测试试验抗体测试试验诊断诊断诊断诊断诊断诊断

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