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在几内亚比绍的儿童中微观和分子识别导致腹的肠道原生虫

Heidi B Bertelsen¹, Signe V Vahlkvist¹, Gitte N Hartmeyer^2,3

¹Department of Pediatrics and Adolescent Medicine, Lillebaelt Hospital, DK-6000 Kolding, Denmark.

Transactions of the Royal Society of Tropical Medicine and Hygiene

|October 10, 2025

概括

像Giardia duodenalis这样的胃肠道寄生虫感染在比绍的儿童中很常见,通常没有症状. 分子检测 (PCR) 比显微镜更准确地诊断这些感染.

科学领域:

传染性疾病传染性疾病
公共卫生公共卫生
分子诊断学分子诊断

背景情况:

胃肠道寄生虫感染在低收入和中等收入国家 (LMICs) 是一个重大的健康挑战.
甲状腺虫 (Giardia duodenalis) 和甲状腺虫 (Entamoeba) 是寄生虫感染的常见原因.

研究的目的:

为了比较显微镜和PCR用于检测儿童胃肠道寄生虫的诊断准确度.
评估寄生虫感染与比绍儿童症状和水源的流行程度和相关性.

主要方法:

通过使用显微镜和PCR检查Giardia duodenalis,Entamoeba物种和Cryptosporidium spp.的1238个儿童便样本.
分析了症状,显微镜强度和PCR周期值 (Ct) 值之间的关联.
根据年龄,性别和居住地对应的孩子.

主要成果:

十二指的患病率很高 (56.2%-63.0%通过PCR),明显高于通过显微镜检测到的.
通过PCR检测显示的特异性高于显微镜检测,这高估了Entamoeba histolytica的流行率.
通过PCR检测的寄生虫与报告的症状没有相关性.

结论:

比绍的儿童表现出相当大的无症状Giardia duodenalis负担.
在诊断寄生虫感染方面,PCR提供了比显微镜更好的特异性.
选择性分子测试与临床评估相结合,建议在资源有限的环境中进行有效治疗.

关键词:

几内亚比绍 - 几内亚比绍这是一个PCRPCR.低收入和中等收入国家.寄生虫感染寄生虫感染寄生虫学的寄生虫学原生动物感染原生动物感染

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