氧同位素分析酸盐和有机化合物通过电子喷射电离轨道轨道轨道质谱仪
Nora M Bernet1,2, Cleo Soldini1, Timo M O Felder3
1Eawag: Swiss Federal Institute of Aquatic Science and Technology, 8600 Dübendorf, Switzerland.
Analytical chemistry
|October 10, 2025
概括
这项研究证明了使用ESI-Orbitrap MS在水样中精确测量酸盐的氧同位素比率. 使用金属有机框架 (MOF) 的新型净化方法可以去除干扰矩阵组件,以进行可靠的生物地球化学和环境分析.
科学领域:
- 分析化学 分析化学
- 地质化学 地质化学
- 环境科学 环境科学
- 生物地质化学生物地质化学
背景情况:
- 电子喷雾电离轨道质谱仪 (ESI-Orbitrap MS) 显示了酸盐和有机化合物中氧同位素比率 (18O/16O) 测量的潜力.
- 之前的研究证实了仪器的可行性,但在多用途质谱仪上的复杂水性矩阵中的适用性仍未得到解决.
- 精确的酸盐测量对于了解代谢,生物地球化学和环境过程至关重要.
研究的目的:
- 评估ESI-Orbitrap MS的长期准确性和精度,用于在水样中确定 δ18O(PO4).
- 为了确定甲醇样品溶液的关键性质,进行精确的测量.
- 开发和提出一个样本净化程序,以消除矩阵干扰.
主要方法:
- 通过ESI-Orbitrap MS.使用H2PO4与PO3-碎片,评估了影响 δ18O(PO4) 的仪器参数.
- 研究了含水量和干扰离子 (酸盐,硫酸盐) 的水性矩阵组成对测量精度的影响.
- 开发并测试了一种使用基于的金属有机框架 (MOF) 进行选择性提取的酸盐净化程序.
主要成果:
- 在两年和10个测量活动中,在±2.8‰内实现了 δ18O(PO4的长期可重现性.
- 在源中碎片化实验 (H2PO4-到PO3-) 显示了对 δ18O 的优异一致,表明有机化合物的潜力.
- 发现高含水量 (>50体积%) 和干扰性氧化离子 (酸盐,硫酸盐) 由于ESI源中的矩阵干扰,限制了准确性.
- 基于MOF的净化程序成功地从复杂的水溶液中提取和回收了酸盐,从而实现了准确的d18O(PO4) 分析.
结论:
- 在水样中,ESI-Orbitrap MS是一种可行的技术,可在适当的样本准备条件下进行精确的 δ18O(PO4) 测量.
- 一种基于MOF的新净化方法有效地消除了矩阵干扰,扩大了这种技术在环境和生物地球化学研究中的适用性.
- 开发的方法促进了酸盐的强大的氧同位素分析,这对于追踪代谢,生物地球化学和环境过程至关重要.
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