高分辨率的滴状细胞动态时间间隔成像通过最佳传输和对比学习
Luca Johannes Schlotheuber1, Michael Vollenweider2, Sven Gutjahr2
1Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, Zürich, Switzerland.
Lab on a chip
|October 10, 2025
概括
我们开发了一种新方法来追踪微流体滴中的细胞,即使有显著的运动. 这一进步改善了单细胞分析,通过长时间的精确细胞追踪.
科学领域:
- 生物技术是生物技术.
- 细胞生物学 细胞生物学
- 微流体学 微流体学
背景情况:
- 单细胞分析对于理解细胞异质性至关重要.
- 微流体滴滴阵列通过时间间隔成像来促进高通量单细胞分析.
- 在微流体液滴中追踪细胞身份是具有挑战性的,因为液滴运动很大,时间间隔很长.
研究的目的:
- 开发一条可靠的管道,用于在很长的距离和时间间隔内追踪微流体滴中的细胞身份.
- 为了克服现有的机器学习方法的局限性,这些方法依赖于标记数据或邻近的细胞.
主要方法:
- 开发了一个管道,将视觉对象检测,特征提取的对比学习和对象匹配的最佳传输相结合.
- 该方法最大限度地减少了对标记训练数据的需求.
- 在实验和模拟条件下得到验证.
主要成果:
- 在很长的距离和很长的间隔 (>30分钟) 上成功跟踪了成千上万的微流体滴.
- 追踪滴滴的成功率达到了>90%,其运动直径高达12滴滴.
- 在超过100滴径的运动中,即使在以前无法追踪的场景中,也证明了超过60%的成功率.
结论:
- 开发的工作流允许在存在大型和复杂的运动模式时准确跟踪微流体滴.
- 这种方法是长期单细胞研究的基础,其中样本的独特性排除了重复实验.
- 增强微流体滴滴阵列用于动态单细胞功能分析的能力.
相关概念视频
01:26Phase Contrast and Differential Interference Contrast Microscopy
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
01:37Super-resolution Fluorescence Microscopy
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.