Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

14.0K
Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
14.0K
Protein-protein Interfaces02:04

Protein-protein Interfaces

14.4K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.4K
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

4.4K
4.4K
Protein and Protein Structure02:15

Protein and Protein Structure

86.8K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
86.8K
Protein and Protein Structures02:15

Protein and Protein Structures

18.6K
18.6K
Conservation of Protein Domains02:26

Conservation of Protein Domains

4.0K
4.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Advances in drug addiction research using <i>Caenorhabditis elegans</i>: behavioral and molecular mechanisms.

Frontiers in cell and developmental biology·2026
Same author

Physical Resilience and Its Influencing Factors Among Older Patients with Fragility Fractures: A Cross-Sectional Study Based on Latent Profile Analysis.

Healthcare (Basel, Switzerland)·2026
Same author

Association Between Depressive Symptoms and Risk of New-Onset Chronic Liver Disease Among Middle-Aged and Elderly Chinese Adults: A Prospective Cohort Study Based on the China Health and Retirement Longitudinal Study.

Healthcare (Basel, Switzerland)·2026
Same author

Synergistic integration of μ-SPE and ammoniated adduct-based MRM for sensitive UPLC-MS/MS quantification of PEG1000: a comparative study against in-source CID and application to zebrafish tissue distribution.

Analytical and bioanalytical chemistry·2026
Same author

Nickel-Catalyzed Cross-Electrophile Couplings of Tetraarylphosphonium Salts with Aryl Bromides.

Organic letters·2026
Same author

Targeted Degradation of STING by a Neutrophil Membrane-Coated Nanoplatform Suppresses Microglial Pyroptosis After Subarachnoid Hemorrhage.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

相关实验视频

Updated: Jan 15, 2026

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
10:58

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

Published on: July 25, 2013

17.6K

GeoEvoBuilder:一个深度学习框架,用于高效的功能和热稳定蛋白质设计.

Jiale Liu1,2, Hantian You3, Zheng Guo1

  • 1Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.

Proceedings of the National Academy of Sciences of the United States of America
|October 10, 2025
PubMed
概括

深度学习框架GeoEvoBuilder通过整合结构和进化数据,直接设计具有增强活性和稳定的蛋白质序列. 这种先进的方法加速了蛋白质工程,绕过了劳动密集型循环.

关键词:
催化效率的催化效率是什么深度学习是一种深度学习.功能性蛋白质的设计序列的新奇性 新奇性热稳定性的热稳定性

更多相关视频

Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins
05:08

Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins

Published on: July 8, 2025

943
Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

7.7K

相关实验视频

Last Updated: Jan 15, 2026

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
10:58

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

Published on: July 25, 2013

17.6K
Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins
05:08

Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins

Published on: July 8, 2025

943
Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

7.7K

科学领域:

  • 生物化学 生物化学
  • 计算生物学 计算生物学
  • 蛋白质工程是指蛋白质工程.

背景情况:

  • 蛋白质工程传统上依赖于代计算设计和实验验证,这是耗时的.
  • 现有的深度学习方法已经推进了蛋白质设计,但很难直接生成具有高活性和稳定性等理想性质的序列.

研究的目的:

  • 介绍GeoEvoBuilder,一个用于直接蛋白序列设计的新型深度学习框架.
  • 为了改进蛋白质工程成果,以适应的方式整合结构和进化约束.

主要方法:

  • 开发了GeoEvoBuilder,这是一个集成结构和进化信息的深度学习框架.
  • 应用 GeoEvoBuilder 来重新设计绿色光蛋白,谷氨过氧化酶 4 (GPX4) 和二叶酸减少酶 (DHFR).
  • 通过实验方法验证设计的蛋白质结构和功能,包括晶体结构的确定.

主要成果:

  • GeoEvoBuilder准确地预测了功能站点,并生成了稳定,活跃的蛋白质序列.
  • 重新设计的DHFR显示,催化效率增加了20倍,温度稳定性提高了10°C.
  • 设计的蛋白质表现出正确的结构,GPX4变体提供了对残留动态相关性和酶活性调节的见解.

结论:

  • GeoEvoBuilder提供了一种对蛋白质工程的变革性方法,通过使直接序列设计能够显著改善活动和稳定性.
  • 该框架成功地重新设计了多种蛋白质,证明了其多功能性和探索大量序列空间的潜力,并进行了大量的残留物修改.
  • GeoEvoBuilder促进了对蛋白质序列-结构-功能-进化关系的更深入的理解,并可供公众研究.