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细胞外ATP是细菌中的环境暗示

Sophie Tronnet¹, Vikash Pandey¹, Miriam Lloret-Berrocal¹

¹The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå 901 87, Sweden; Umeå Centre for Microbial Research (UCMR), Umeå 901 87, Sweden; Department of Molecular Biology, Umeå University, Umeå 901 87, Sweden.

|October 10, 2025

概括

细胞外ATP (eATP) 向细菌发出信号,改变它们的新陈代谢,抗生素耐药性和毒性. 这项研究揭示了eATP是影响 prokaryotic 生理学和宿主相互作用的关键环境提示.

科学领域:

微生物学微生物学
生物化学生物化学
分子生物学分子生物学

背景情况:

细胞外ATP (eATP) 是动物和植物中已知的信号分子,调节免疫反应.
肠道细菌在炎症期间遇到eATP水平升高,但它们对它的反应在很大程度上是未知的.

研究的目的:

研究细菌,特别是大肠杆菌是否对细胞外ATP (eATP) 有反应.
阐明细菌对eATP的反应背后的分子和代谢机制.
确定eATP信号在细菌中的功能后果,包括对抗菌素耐药性和毒性的影响.

主要方法:

利用基因组规模的促进器库来评估eATP依赖的基因调节.
运用代谢学来分析细菌代谢物概况的变化.
综合基因组规模建模以了解全球代谢转变.
测试了eATP对细菌对抗生素和抗微生物的敏感性的影响.

主要成果:

大肠杆菌表现出对eATP的时间,度和介质依赖的ATP特异性反应.
eATP调节了第二信使通路,并调节了参与新陈代谢,生物膜形成和信封应激的基因.
代谢和建模分析揭示了细菌代谢网络和生物质组成的显著变化.
细菌接触eATP会影响对抗生素和抗菌的敏感性.
在致病性细菌中,eATP被发现可以控制毒性因子的表达.

结论:

细胞外ATP (eATP) 作为 prokaryotes 的一个重要的环境提示,广泛调节他们的生理学.
细菌对eATP的反应会影响抗微生物耐药性和毒性,这表明它们在宿主微生物相互作用中的作用.
细菌中的eATP信号代表了一种新的调节机制,对了解微生物生态和病原体产生有影响.

关键词:

CP: 微生物学微生物学它们的属性是 Enterobacteriaceae.抗微生物耐药性抗微生物耐药性细胞外的ATP 基因表达的基因表达方式这是一种炎症炎症炎症炎症.肠道细菌是一种肠道细菌.代谢产物的代谢产物生理学生理学生理学纯能信号传递是纯能信号.毒性毒性是一种毒性.

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