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精子制备技术会影响生物参数,生育指数和DNA碎片化在患有oligo-astheno-teratozoospermia的患者

Banafsheh Heidari¹, Amin Nosrati², Mostafa Pournourali³

¹Regenerative Medicine and Biotechnology in Wound Healing, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran.

Reproduction & fertility

|October 10, 2025

概括

最好的精子制备方法取决于患者的病情. 密度梯度离心 (DGC) 对于奥利戈-阿斯-类精子症 (OAT) 患者来说是最佳的,而白蛋白梯度过 (AGF-2) 和上游对于常态精子症来说更好,在所有情况下都能减少DNA损伤.

科学领域:

生殖生物学生殖生物学
安德罗学与人类学
精子加工技术精子加工技术

背景情况:

精子制备对于辅助生殖技术至关重要.
确定不同精子质量配置文件的最佳技术是必不可少的.
在精子选择方面,Oligo-astheno-teratozoospermia (OAT) 和normozoospermia存在着不同的挑战.

研究的目的:

为了评估密度梯度离心 (DGC),白蛋白梯度过 (AGF-1,AGF-2) 和上游技术的有效性.
为了比较它们对精子生物参数,染色质完整性和DNA碎片化的影响.
为了确定最适合OAT和normzoospermic患者的精子制备方法.

主要方法:

一项涉及100名男性的比较研究 (OAT和normzoospermia).
评估精子参数 (度,运动性,形态) 和特定生育指数 (SSFI).
使用SQA-V Gold,TUNEL和COMET测定进行染色体完整性和DNA碎片化的评估.

主要成果:

在OAT患者中,DGC产生了最多的运动精子,具有最佳速度和SSFI.
而AGF-2在常态种子患者中被证明最有效.
在不同患者群体中,DGC,AGF-2和上游显著减少了精子DNA损伤.

结论:

精子制备技术应根据个体患者的个人资料进行量身定制.
DGC是OAT患者的首选方法,提高精子质量和减少DNA损伤.
AGF-2和上游更适合于常态种类的个体,优化精子选择和DNA完整性.

关键词:

生育能力生育能力生育能力生育能力形态学形态学形态学常态动物精子症 (normozoospermia) 是一种常态动物精子症.精子制备技术精子制备技术速度的速度是速度的速度.

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