相关实验视频

Updated: Jan 15, 2026

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

全基因组的CRISPR屏幕识别了剪接因子SF3B4在驱动肝细胞癌中的作用

Yue Guo¹, Mingjing Xu¹, Hua Xue¹

¹Department of Surgery, Sir Y.K. Pao Centre for Cancer, The Chinese University of Hong Kong, Shatin, Hong Kong, China.

Science advances

|October 10, 2025

概括

剪切酶因子SF3B4对于肝细胞癌 (HCC) 存活率和伦瓦提尼布耐药性至关重要. 这项研究确定SF3B4是HCC进展和铁灭菌耐药性的关键驱动因素.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

基因组测序已经在肝细胞癌 (HCC) 中确定了与癌症相关的基因,但它们的功能作用往往不清楚.
了解基因功能对于开发针对性治疗HCC至关重要.

研究的目的:

通过全基因组的CRISPR淘汰查,在HCC中识别必要的生存基因.
调查结合体因子和铁灭调节器在HCC和伦瓦提尼布耐药性的作用.

主要方法:

在HCC有机体中进行全基因组CRISPR淘汰选.
对结合酶因子和铁灭抑制剂的分析,包括谷氨酸氨酸酶催化子单元 (GCLC).
RNA免疫沉测序,长读异型测序和转录组分析以识别SF3B4标.

主要成果:

剪切酶因子对于HCC细胞存活至关重要.
SF3B4被确定为排名第一的基因,促进HCC有机体的存活率和体内瘤的发生.
SF3B4调节了一个特定的拼接环境,T-box转录因子3 (TBX3) 变异TBX3+2a被确定为下游效应因子.
像GCLC这样的铁灭抑制剂的升调在耐伦瓦替尼布的HCC中被观察到.

结论:

SF3B4在HCC细胞存活和瘤进展中起着至关重要的作用.
SF3B4涉及那些对伦瓦提尼布无反应的患者的铁死耐药性.
向SF3B4可能为林瓦替尼抗性HCC提供治疗策略.

更多相关视频

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

相关概念视频

Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...