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相关概念视频

RNA Splicing01:32

RNA Splicing

60.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.3K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

6.6K
6.6K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

3.3K
3.3K
Alternative RNA Splicing02:18

Alternative RNA Splicing

24.7K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.7K
Alternative RNA Splicing02:18

Alternative RNA Splicing

4.8K
4.8K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

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U2 snRNP recognizes the branch site through a loaded-spring strand-invasion mechanism.

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Structural flexibility of the human vault particle revealed by high-resolution cryo-EM and molecular dynamics simulations.

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Combined All-Atom Simulations and Biophysical Assays Uncover Loop-Driven Stabilization in the HRAS i-motif.

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Exploring Disordered Regions of Human Spliceosome Proteins.

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相关实验视频

Updated: Jan 15, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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解码Spliceosome动力学通过计算和实验.

Pavlína Pokorná1, Jana Aupič1, Sebastian M Fica2

  • 1CNR-Istituto Officina dei Materiali (IOM) at International School for Advanced Studies (SISSA/ISAS), via Bonomea 265, 34136 Trieste, Italy.

Chemical reviews
|October 10, 2025
PubMed
概括

拼接体,一个动态的RNA-蛋白质机器,通过复杂的重塑来协调RNA拼接. 整合实验和计算方法提高了我们对其功能的理解,并有助于开发新疗法.

科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学
  • 结构生物学 结构生物学

背景情况:

  • 拼接体是一个动态的RNA蛋白机器,负责RNA拼接.
  • 它的功能依赖于在拼接周期期间的大规模结构和构成改造.
  • 低温EM提供结构快照,但缺乏动态细节.

研究的目的:

  • 通过整合实验和计算方法来审查目前对结合体功能的理解.
  • 突出拼接因子在拼接体动态中的作用.
  • 讨论计算挑战和准确模拟的未来方向.

主要方法:

  • 对现有文献的审查,将实验数据 (cryo-EM) 与计算模拟 (全原子模拟) 整合在一起.
  • 专注于计算方法来捕捉spliceosome的动态性质.
  • 讨论大规模模拟中的挑战.

主要成果:

  • 全原子模拟通过在更细微的尺度上捕捉拼接体动态来补充冷EM.
  • 拼接因子是拼接体的动态行为的关键调解者,对周期进展至关重要.
  • 实验和计算之间的协同作用对于高精度结构合集至关重要.

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Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
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相关实验视频

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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
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结论:

  • 综合性方法对于全面了解合体机制至关重要.
  • 这种方法有望在聚合向治疗和基因调制技术方面取得进展.
  • 解决疾病中的拼接失调需要结合计算和实验数据.