在基因工程Aspergillus oryzae中通过驱动器形状和pH控制来增强isoprimeverose产生酶,用于大规模发酵
Fahmi Baihaqqi1, Satoshi Wakai2, Filemon Jalu Nusantara Putra1
1Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-Ku, Kobe, Hyogo 657-8501, Japan.
Enzyme and microbial technology
|October 10, 2025
概括
这项研究优化了Aspergillus oryzae的发酵,以增加罕见的酶异原酶 (IpeA) 的产量. 马尔托介质和特定的生物反应器条件显著提高了潜在商业应用的酶产量.
科学领域:
- 生物技术是生物技术.
- 酶工程是什么? 酶工程是什么?
- 工业微生物学 工业微生物学
背景情况:
- Aspergillus oryzae产生有价值的多糖分降解酶.
- 伊索普里梅弗洛斯氧化糖酶 (IpeA) 对于释放罕见的糖伊索普里梅弗洛斯至关重要.
- 目前,IpeA的商业可用性有限.
研究的目的:
- 通过发酵工程来增强IpeA的生产.
- 为了优化发酵参数以提高IpeA产量.
- 为了研究碳源,pH值和驱动器类型对A. oryzae种植的影响.
主要方法:
- 的发酵工程 Aspergillus oryzae.
- 优化碳源 (葡萄糖与麦芽糖) 和pH值.
- 在5L生物反应器中,盘轮 (DT) 和MAXBLEND® (MB) 螺旋的比较.
主要成果:
- 与葡萄糖相比,马尔托斯补充剂减少了碳催化剂的抑制.
- 酸性pH (4.5) 和MB驱动器最大化了生物质和IpeA产量 (∼19 g/L DMW,∼68 U/mL IpeA).
- 在测试条件下,MB螺旋显著超过了DT螺旋的性能.
结论:
- 优化的发酵参数显著提高了A. oryzae中的IpeA产量.
- 作为碳源的马尔托斯和特定的生物反应器配置是高产量的关键.
- 这些发现为可扩展的A. oryzae和IpeA.生物生产提供了基础.
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