Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.1K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.1K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

20.5K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
20.5K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

6.5K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
6.5K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.3K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.3K
Mass Spectrometry: Molecular Fragmentation Overview01:20

Mass Spectrometry: Molecular Fragmentation Overview

5.4K
The ionization of a molecule into a molecular ion inside the mass spectrometer causes instability in the molecule's structure due to the loss of an electron. This eventually leads to the fragmentation or breaking of some bonds in the molecule. The fragmentation occurs predominantly at specific bonds to yield relatively stable fragments.
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can occur at...
5.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Fungal Extracellular Vesicles are Recoverable Across Variable Ultracentrifugation Speeds but Display Species-specific Profiles of Sedimentation.

The Journal of membrane biology·2026
Same author

Cellular and molecular responses of <i>Cryptococcus</i> to brilacidin.

Microbiology spectrum·2026
Same author

Single-cell proteomics: When individual cells tell population stories.

Trends in biochemical sciences·2026
Same author

Comparative omics reveals conserved extracellular matrix signatures during mammary tumor progression.

Frontiers in oncology·2026
Same author

Quinoa bioester application shifts human skin proteome toward molecular profiles associated with younger age.

Communications biology·2026
Same author

Human milk oligosaccharides promote synaptogenesis and neurite outgrowth in human cortical organoids.

Biochemistry and biophysics reports·2026

相关实验视频

Updated: Jan 15, 2026

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS
17:12

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS

Published on: December 20, 2010

16.0K

序列组装器:一种用于从质谱数据中组装蛋白质序列的工具.

Celso Vitor A Q Calomeno1, Hulyana Brum2, Rodrigo S C Brant3

  • 1Laboratory for Structural and Computational Proteomics, Carlos Chagas Institute, Fiocruz Paraná, Paraná, Brazil.

Journal of proteomics
|October 10, 2025
PubMed
概括

序列组合器 (SA) 软件集成了谱匹配和de novo测序数据,以重建全长蛋白质. 这个用户友好的工具增强了用于生物研究和生物制药开发的蛋白质测序.

关键词:
生物信息学是一种生物信息学.质谱测量质量谱测量蛋白质测序组件组件蛋白质组学是指蛋白质组学.

更多相关视频

Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

9.1K
Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

845

相关实验视频

Last Updated: Jan 15, 2026

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS
17:12

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS

Published on: December 20, 2010

16.0K
Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

9.1K
Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

845

科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 生物技术是生物技术.
  • 生物制药开发 生物制药开发

背景情况:

  • 完整蛋白质的精确测序对于理解生物系统和推动药物开发至关重要.
  • 目前的方法可能缺乏全面的蛋白质序列组合的整合.

研究的目的:

  • 介绍SequenceAssembler (SA),一个用于组装全长蛋白质序列的软件工具.
  • 整合谱匹配 (PSM) 和 de novo 测序数据以增强蛋白质重建.

主要方法:

  • 开发了具有用户友好的图形界面的SequenceAssembler (SA) 软件.
  • 集成SA与已建立的蛋白质组学工具 (Novor Cloud,PEAKS Studio,蛋白质组学模式实验室).
  • 通过使用高分辨率质谱学分析牛血清白蛋白和trastuzumab来验证SA.

主要成果:

  • 通过结合PSM和de novo测序数据,SA成功组装了全长蛋白质序列.
  • 性能与Stitch等现有工具相当,结果取决于参数值.
  • 经过简化工作流程和一键安装的使用方便.

结论:

  • SequenceAssembler为蛋白质序列组装提供了一个统一的平台,解决了蛋白质组学中的差距.
  • 直观的界面降低了采用障碍,提高了核心实验室的可复制性和效率.
  • SA促进了各种研究应用的快速蛋白质测序.